Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

684274 β1,2-Xylosidase, Xanthomonas sp.

View Products on Sigmaaldrich.com
684274
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
684274-500MIU Plastic ampoule 500 miu
Description
OverviewNative β1,2-Xylosidase from Xanthomonas species. Catalyzes the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of α1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.
Note: 1 mU = 1 milliunit.
Catalogue Number684274
Brand Family Calbiochem®
Synonymsβ-D-Xylanxylohydrolase
References
ReferencesReese, A.J. and Doening, T.L. 2003. Mol. Microbiol. 50, 1401.
Product Information
CAS number9025-53-0
Activity≥10 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will release 1 nmol of methylumbelliferone from 4-methylumbelliferyl-7-β-D-xyloside per min at 37°C, pH 5.0.
EC number3.2.1.37
FormLiquid
FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥20 units/mg protein
Physicochemical Information
Contaminantsβ-N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α-mannosidase, proteases: none detected
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Special InstructionsFollowing initial use, refrigerate (4°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
684274-500MIU 04055977225358

Documentation

β1,2-Xylosidase, Xanthomonas sp. SDS

Title

Safety Data Sheet (SDS) 

β1,2-Xylosidase, Xanthomonas sp. Certificates of Analysis

TitleLot Number
684274

References

Reference overview
Reese, A.J. and Doening, T.L. 2003. Mol. Microbiol. 50, 1401.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision16-September-2008 RFH
Synonymsβ-D-Xylanxylohydrolase
DescriptionNative β1,2-Xylosidase from Xanthomonas species. Catalyses the hydrolysis of terminal xylose β1,2-linked to the β-mannose of N-linked oligosaccharides. Enzyme requires removal of a1,3-linked mannose. Enzyme requires 5 mM calcium chloride in reaction buffer for full activity.
FormLiquid
FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.
Recommended reaction conditionsRecommended reaction buffer: 50 mM sodium acetate buffer, 5 mM CaCl2, pH 5.0.
CAS number9025-53-0
EC number3.2.1.37
Contaminantsβ-N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α-mannosidase, proteases: none detected
Specific activity≥20 units/mg protein
Activity≥10 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will release 1 nmol of methylumbelliferone from 4-methylumbelliferyl-7-β-D-xyloside per min at 37°C, pH 5.0.
Storage +2°C to +8°C
Do Not Freeze Yes
Special InstructionsFollowing initial use, refrigerate (4°C).
Toxicity Standard Handling
ReferencesReese, A.J. and Doening, T.L. 2003. Mol. Microbiol. 50, 1401.