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Life Science Research
 

Amicon® Pro Purification System - Protein Purification

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Introducing an all-in-one centrifugal device for the protein purification workflow. Traditional protein purification is a long process with many steps and multiple tubes, often resulting in protein degradation and loss. Avoid the risks associated with sample transfer and reduce hands-on time when you bind, wash, elute and/or concentrate your protein in the all-in-one Amicon® Pro purification system. No matter what your workflow, this virtually touchless device delivers consistent, accurate sample preparation, resulting in more reliable recovery, uncompromised purity and easier data generation.

Download our application note on Condensing the Protein Purification Workflow

The Amicon® Pro Purification System Workflow

Bind, wash, elute and/or concentrate your protein in this all-in-one centrifugal device.

Amicon® Pro purification system workflow

Elute, Don't Dilute

Excellent Recovery, Regardless of Scale
Elute, don't dilute with the new Amicon® Pro Purification System
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While three elution steps were required for the smaller spin columns (left), a single spin in the Amicon® Pro device was sufficient to recover > 90% of resin-bound protein in an average of 45 µL final volume*.
Excellent Recovery with new Amicon® Pro Protein Purification workflow
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Protein recovered increases linearly with respect to packed resin volume, offering flexibility in experimental scale. Whether you are using a few microliters or a milliliter of resin, Amicon® Pro purification yields consistent recovery.

Compare the Amicon® Pro Workflow with Traditional Methods

 
Purification with buffer exchange/concentration
Purification only
0.5 mL spin column + concentration device Amicon® Pro Purification System 0.5 mL gravity column Amicon® Pro Purification System
Process time 67 minutes 29 minutes 125 minutes 65 minutes
Steps required 12 6 18 6
Sample transfers 1 0 3-8 (# of fractions collected) 0
1 Process time excludes 60 min incubation for both protocols.
2 Gravity column process includes column setup, purification and assessment of eluted fractions.