Amicon® Ultra Diafiltration |
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Amicon® Ultra centrifugal devices are ideal for removal or exchange of salts, sugars, nucleotides, and non-aqueous solvents, as well as other materials of low molecular weight. They also serve to separate free from bound species.
Amicon® Ultra centrifugal concentrators provide fast, convenient, high-recovery alternatives to dialysis and ethanol precipitation. Sample dilution, often associated with spin columns, is not a problem. Salt transfer across the membrane is efficient and independent of microsolute concentration or size.
Advantages:
- Fast: buffer exchange in as few as two spins
- Efficient: requires minimal volume of exchange buffer, easily contained in reservoir
- Easy to use: simply load your sample with standard pipette
- Enables simultaneous concentrating and desalting
Efficient diafiltration: Follow these steps to use an Amicon® Ultra 0.5 mL centrifugal filter to concentrate a protein sample 10X while also gradually reducing the salt concentration by 9X.