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70088 pBAC™-3 DNA - Novagen

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70088
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70088-3
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      Description
      OverviewpBAC™-3 is a baculovirus transfer plasmid compatible with BacVector™-1000, -2000, or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter and expression of secreted proteins. A 20 amino acid gp64 signal peptide sequence is capable of directing high levels of protein into the secretory pathway of infected insect cells. Cloning sites are provided for the creation of N-terminal fusions of an insert with His•Tag® and/or S•Tag™ sequences. pBAC-3 provides an ATG start codon at the optimal position relative to native polyhedrin translation signals. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.





      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Catalogue Number70088
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      70088-3 04055977258707

      Documentation

      pBAC™-3 DNA - Novagen FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      pBAC™-3 DNA - Novagen Certificats d'analyse

      TitreNuméro de lot
      70088

      Brochure

      Titre
      Insect Cell Expression

      Citations

      Titre
    • Hyesook Yoon, et al. (2007) Activation profiles and regulatory cascades of the human kallikrein-related peptidases. Journal of Biological Chemistry 282, 31852-31864.
    • Carte des vecteurs

      Titre
      TB217 pBAC™-3 Vector Map

      Séquence du vecteur

      Titre
      pBAC-3 Sequence

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      Catégories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Insect Expression > Insect Expression Vectors & Kits