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Unités de filtration Steritop™

Les unités de filtration Steritop offrent une grande surface de filtration permettant une filtration stérilisante rapide de liquides aqueuxl.

Les unités de filtration Steritop offrent une grande surface de filtration permettant une filtration stérilisante rapide de liquides aqueux dans des flacons à petit (33 mm) ou grand (45 mm) col. Un petit ergot à l'intérieur de l'entonnoir maintient le préfiltre (en option) en place pendant la filtration. Pour une meilleure protection contre la contamination, le filetage s'adaptant au flacon est légèrement en retrait au cas où l'entonnoir toucherait accidentellement le plan de travail.

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Caractéristiques

Guide d'achat

Unités avec membrane Millipore Express PLUS (PES) 0,22 µm à débit élevé et faible adsorption protéique pour la stérilisation des solutions aqueuses*Supprimer le tri & les filtres Show Filter
RéférenceDescriptionCapacité de l'entonnoirRaccord de sortieConditionnement
SCGPS01REUnité de filtration pour flacon fonctionnant sous vide 150 mL Filetage 33 mm 12 Prix & Disponibilité
SCGPS02RESteritop-GP, 0,22 µm, polyéthersulfone, 250 ml, 33 mm, radio-stérilisé 250 mL Filetage 33 mm 12 Prix & Disponibilité
SCGPS05RESteritop-GP, 0,22 µm, polyéthersulfone, 500 ml, 33 mm, radio-stérilisé 500 mL Filetage 33 mm 12 Prix & Disponibilité

* Ces produits ont été testés et leur utilisation validée pour les applications de recherche sur les cellules souches. Afin de déterminer leurs effets sur la croissance et la différenciation de cellules souches de souris, trois lots d'unités Steritop-GP ont été utilisés pour filtrer les milieux avec le facteur LIF (Leukemia Inhibiting Factor). Une fois filtré le milieu a été utilisé pour passer les cellules souches de souris cinq fois afin de vérifier que les unités de filtration Steritop-GP n'affectent pas la pluripotence des cellules souches de souris.

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Documentation

FAQ

QuestionRéponse
Are Stericup and Steritop units autoclavable?No. The highest temperature that the product can be used at is 50C ( 122F ).
Can I bubble point my Stericup/top?No. The unit is not designed to handle the pressures required to perform a bubble point test. It is designed to be run under vacuum only (15 psi max). Bubble points for any of the membranes in the Stericups/tops need to be run at pressures ranging from 22 psi to 57psi.
Do you have a filter for 100% serum?Yes, you can filter serum with Millipore Express membrane. The Express membrane was designed for this application and offers fast filtration time. Millipore Express is available in a large number of filter devices including Millex syringe filters, Stericup and Steritop vacuum devices, Sterivac for large volumes of media and Steriflip for 50 mls.
For tissue culture media filtration which membrane should I use?The best membrane for this application is the Millipore Express membrane. It was designed specifically to filter tissue culture media with serum. It is low protein binding, has fast flow and high throughput (which is also called filter capacity).
I want to sterilize 100% ethanol. Are any of the Stericups or Steritops compatible with ethanol?The funnel and receiver flask is made of polystyrene. Any molded polystyrene will craze in pure ethanol and could structurally fail depending on the forces being applied to it. The funnel could handle the short exposure time but the receiver would craze eventually. Therefore we would not recommend the Stericup with 100% ethanol. The Steritop, if you keep the exposure time to the funnel minimal, should be fine. As you dilute the alcohol the compatibility improves.

Both Express and Durapore membranes are compatible. MCE is not.
I will be filtering tissue culture media with your Stericup/top and am concerned that wetting agents in the filter could effect my cells. Which membrane type would you recommend to avoid this?All of Millipore's Tissue Culture Media filtration sterilization devices contain membranes that are wetting agent (surfactant ) free.
Is the Express membrane surfactant free?Yes, the Express membrane is surfactant free. It also has a very low extractables specification - less then 1.5% by weight.
When I use my Stericup/top, sometimes some of my sterilized solution gets pulled into my vacuum line. How can I eliminate this from happening?Attach your vacuum tubing and turn the vacuum on before adding the liquid to the Stericup/top. This eliminates any initial solution that flowed through the membrane before starting the vacuum from being pulled into the vacuum line.
What is the diameter of the membrane in the Stericup and Steritop units?All of the cups and tops currently have a 70mm diameter membrane, which has 43 cm2 of surface area.
Why use a vacuum driven filter rather than a pressure driven filter?Unlike positive pressure filtration, vacuum driven filtration requires only a vacuum source. It does not require pressure vessels or peristaltic tubing pumps. Stericup and Steritop units are pre-sterilized and disposable making setup and cleanup easy!
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Steritop™

Catégories

Life Science Research > Cell Culture and Systems > Sterile Filtration Devices