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03-0185-00 SMC™ Human Total Tau High Sensitivity Immunoassay Kit

The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human Total Tau High Sensitivity Immunoassay Kit unparalleled performance in the quantification of Human Total Tau in human serum, plasma, and CSF.

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Key Applications: Single Molecule Counting (SMC®) Research Category: Neuroscience, neurological Research Sub-Category: Alzheimer's Disease, Aging, Nervous System, Neurodegenerative Diseases, Neurofilament & Neuron Metabolism, Neuronal & Glial Markers, Neuroregenerative Medicine, Neuroscience
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03-0185-00
96 well assay  
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      Replacement Information

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      Key Applications
      Single Molecule Counting (SMC®)
      Description
      Catalogue Number03-0185-00
      Trade Name
      • SMC®
      DescriptionSMC™ Human Total Tau High Sensitivity Immunoassay Kit
      OverviewThe SMC™ Human Total Tau High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
      Background InformationThe human Tau family of microtubule-associated neuronal proteins has been rigorously studied in the context of normal and aberrant neurobiology. While typically involved in the stability of axonal microtubules, Tau proteins can also play a role in neurodegenerative diseases such as Alzheimer’s Disease (AD) and frontotemporal dementia (FTD) if they aberrantly misfold and accumulate in the brain. There is significant interest in studying the level of Tau proteins in both the central nervous system as well as in circulation, and the new SMC™ Human Total-Tau High Sensitivity Immunoassay Kit enables researchers to take advantage of the Single Molecule Counting ultrasensitive immunoassay technology to perform accurately and sensitive measurements in human cerebrospinal fluid (CSF), plasma, and serum samples, toward making important discoveries about the role of Tau protein in neurodegenerative disease mechanisms.
      References
      Product Information
      Components
      • Assay Buffer
      • Total Tau Coated Beads
      • Standard Diluent
      • Total Tau Detection Antibody
      • Total Tau Standard
      • 10X Wash Buffer
      • Buffer D
      • Elution Buffer B
      Precision, %
      • The assay variations of SMC™ Human Total Tau Immunoassay kit were studied using five normal plasma samples run in triplicate by 3 different operators on 3 different days.
      • Intra-assay CV% average : ≤ 8%
      • Inter-assay CV% average : ≤ 4%
      Applications
      ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human Total Tau High Sensitivity Immunoassay Kit unparalleled performance in the quantification of Human Total Tau in human serum, plasma, and CSF.
      Key Applications
      • Single Molecule Counting (SMC®)
      Application NotesThis new streamline kit has the following features:
      • Improved options for sample prep and incubation
      • Lyophilization protein standard for uniform shipping and storage condition
      • Increased reagent volumes to accommodate automation
      • Able to achieve assay specifications on both SMCxPRO™ and Erenna®.
      Biological Information
      Species Reactivity NoteSpecificity to the following species samples were tested with the follow results:
      Non Human Primate – 50%
      Mouse – 100%
      Rat – 100%
      Canine – 100%
      Feline – 75%
      Cross ReactivityCross-reactivity was not tested.
      Accuracy
      • Minimum of 3 serum samples spiked with average recovery of 70% with a range of 64-80%
      • Minimum of 4 plasma samples spiked with average recovery of 82% with a range of 76-88%
      • Minimum of 5 CSF samples spiked with average recovery of 91% with a range of 85-106%
      Physicochemical Information
      Sensitivity
      • Lower Limit of Quantification: 4.69 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      03-0185-00 04061841903789