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03-902 RIPAb+™ GFP - RIP Validated Antibody and Primer Set

This RIPAb+™ GFP -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

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Research Category: Epigenetics & Nuclear Function
03-902
1 Kit  
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      Species Reactivity
      Vrt
      Description
      Catalogue Number03-902
      DescriptionRIPAb+™ GFP - RIP Validated Antibody and Primer Set
      Background InformationThe Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
      References
      Product Information
      Components
      • Anti-Green Fluorescent Protein Antibody: Part No. AB3080.
        Rabbit IgG purified: Part No. PP64B.
        RIP Primers, U1 snRNA: Part # CS203215
      FormatPurified
      PresentationAnti-Green Fluorescent Protein Antibody: One vial containing 50 μg of protein G purified antibody in 50 μL buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4). Store at -20°C.
      Normal Mouse IgG: One vial containing 125 μg purified mouse IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
      RIP Primers, U1 snRNA : One vial containing 75 μL of 5 μM of each primer specific for the cDNA of human U1 snRNA. Store at -20°C.
      FOR: GGG AGA TAC CAT GAT CAC GAA GGT
      REV: CCA CAA ATT ATG CAG TCG AGT TTC CC
      Quality LevelMQ200
      Applications
      ApplicationThis RIPAb+™ GFP -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
      Application NotesRNA Binding Protein Immunoprecipitation:
      RIP Lysate prepared from HeLa cells (1 X 10E7 cell equivalents per IP) expressing SNRNP70-GFP-FLAG (Cat. No. 03-903) by RNA transfection was subjected to immunoprecipitation using 5 µg of either a normal mouse IgG or Anti-Green Fluorescent Protein Antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of SNRNP70-GFP-FLAG associated RNA was verified by qPCR using RIP Primers, U1 snRNA.

      Immunoprecipitation:
      RIP Lysate prepared from HeLa cells (1 X 10E6 cell equivalents per IP) expressing SNRNP70-GFP-FLAG (Cat. No. 03-903) by RNA transfection was subjected to immunoprecipitation using 0.5 µg of either a normal rabbit IgG, or anti-Green Fluorescent Protein Antibody. Precipitated proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Green Fluorescent Protein Antibody (1.0 µg/mL). Proteins were visualized using Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP (Cat. # AP187P) and a chemiluminescence detection system.
      Biological Information
      ImmunogenHighly purified native GFP from Aequorea victoria
      ConcentrationPlease refer to datasheet
      HostRabbit
      SpecificityThe anti-Green Fluorescent Protein Antibody is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the antibody has been purified by Protein A chromatography, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli.
      IsotypeIgG
      Species Reactivity
      • Vertebrates
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • GFP
      • eGFP
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightVarious
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRNA Binding Protein Immunoprecipitation: RIP Lysate prepared from HeLa cells (1 X 10E7 cell equivalents per IP) expressing SNRNP70-GFP-FLAG (Cat. No. 03-903) by RNA transfection was subjected to immunoprecipitation using 5 µg of either a normal mouse IgG or Anti-Green Fluorescent Protein Antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of SNRNP70-GFP-FLAG associated RNA was verified by qPCR using RIP Primers, U1 snRNA.
      Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
      Packaging Information
      Material Size1 Kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      03-902 04065265138673