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676604 PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6)

This PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer¹⁵⁷).

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Vasodilator-stimulated Phosphoprotein, Activated

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676604
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Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Prix & Disponibilité

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676604-100UG
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      Description
      OverviewRecognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
      Catalogue Number676604
      Brand Family Calbiochem®
      SynonymsAnti-Vasodilator-stimulated Phosphoprotein, Activated
      Application Data
      Detection of human VASP, phosphorylated on Ser157, by immunoblotting. Samples: Whole cell extracts from human platelets cells left untreated (lane 1) or SNP treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer157) Mouse mAb (5C6) (Cat. No. 676604) (0.5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesBear, J.E., et al. 2001. Curr. Opin. Cell. Biol. 13, 158.
      Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol. 166, 5550.
      Burkhardt, M., et al. 2000. J. Biol. Chem. 275, 33536.
      Harbeck, B., et al. 2000. J. Biol. Chem. 275, 40.
      Smolenski, A., et al. 2000. J. Biol. Chem. 275, 25723.
      Product Information
      FormLyophilized
      FormulationLyophilized from PBS, PEG, sucrose.
      Positive controlVanadate-treated A431 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesELISA (0.05 µg/ml)
      Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
      Immunocytochemistry (1-10 µg/ml)
      Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
      Application CommentsDoes not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP
      ImmunogenHuman
      Clone5C6
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      676604-100UG 04055977260564

      Documentation

      PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) Certificats d'analyse

      TitreNuméro de lot
      676604

      Références bibliographiques

      Aperçu de la référence bibliographique
      Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol. 13, 158.
      Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol. 166, 5550.
      Burkhardt, M., et al. 2000. J. Biol. Chem. 275, 33536.
      Harbeck, B., et al. 2000. J. Biol. Chem. 275, 40.
      Smolenski, A., et al. 2000. J. Biol. Chem. 275, 25723.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-September-2009 JSW
      SynonymsAnti-Vasodilator-stimulated Phosphoprotein, Activated
      ApplicationELISA (0.05 µg/ml)
      Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
      Immunocytochemistry (1-10 µg/ml)
      Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
      Application Data
      Detection of human VASP, phosphorylated on Ser157, by immunoblotting. Samples: Whole cell extracts from human platelets cells left untreated (lane 1) or SNP treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer157) Mouse mAb (5C6) (Cat. No. 676604) (0.5 µg/ml). Detection: chemiluminescence.
      DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
      BackgroundVASP (vasodilator-stimulated phosphoprotein), an established substrate of cAMP- and cGMP-dependent protein kinases in vitro and in living cells, is associated with focal adhesions, microfilaments, and membrane regions of high dynamic activity. VASP binds in vitro to profilin, zyxin, vinculin, and the Listeria sp. surface protein ActA. VASP also promotes actin nucleation and binds to actin filaments in vitro and associates with stress fibers in cells. Studies imply that VASP phosphorylation may act as an immediate negative regulator of actin dynamics. Phosphorylation of Ser157 in VASP can be used as a measure of PKA activity.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP
      Clone5C6
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlVanadate-treated A431 cells
      FormLyophilized
      FormulationLyophilized from PBS, PEG, sucrose.
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesBear, J.E., et al. 2001. Curr. Opin. Cell. Biol. 13, 158.
      Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol. 166, 5550.
      Burkhardt, M., et al. 2000. J. Biol. Chem. 275, 33536.
      Harbeck, B., et al. 2000. J. Biol. Chem. 275, 40.
      Smolenski, A., et al. 2000. J. Biol. Chem. 275, 25723.

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      Catégories

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