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14-503M PKCβI Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant amino acids 2-end of human PKCβ. For use in Kinase Assays.

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14-503M
250 µg  Also available in 10 μg size (cat#14-503) and in bulk (cat# 14-503-K).
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      Replacement Information
      Description
      Catalogue Number14-503M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPKCβI Protein, active, 250 µg
      OverviewN-terminal His6-tagged, recombinant amino acids 2-end of human PKCβ.

      PKC Lipid activator (Catalog # 20-133) is required for use.
      References
      Product Information
      Quality LevelMQ300
      Applications
      ApplicationActive, N-terminal His6-tagged, recombinant amino acids 2-end of human PKCβ. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceHuman PKCβ expressed in Sf21
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
      Gene Symbol
      • PRKCB1
      • PRKCB
      • PRKCB2
      • PKCB
      • MGC41878
      • PKC-B
      • PKC-beta
      Protein TargetPKCβI
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05771 # This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May be considered as a novel component of the NF-kappa-B signaling axis responsible for the survival and activation of B-cells after BCR cross-linking (By similarity).
      COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).
      SIZE: 671 amino acids; 76869 Da
      SUBUNIT: Interacts with PDK1 (By similarity). Interacts in vitro with PRKCBP1.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity).
      PTM: Phosphorylation on Thr-500 of isoform beta-I, within the activation loop, renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Similarly, isoform beta-II is autophosphorylated on 'Thr-640' and 'Ser-659', subsequent to phosphorylation on Thr-500. Autophosphorylated on other sites i.e. in the N-terminal and hinge regions have no effect on PKC activity (By similarity).
      SIMILARITY: SwissProt: P05771 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
      Molecular Weight78kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of Histone H1
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-503) and in bulk (cat# 14-503-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      14-503M 04053252407345

      Documentation

      Produits & Applications associés

      Alternative Packsize

      Référence Description  
      14-503 PKCβI Protein, active, 10 µg Prix & Disponibilité

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      Catégories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases