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SCC448 OSUMMER.4 Mouse NRAS-Mutant Melanoma Cell Line

The OSUMMER.4 melanoma cell line carries the NRAS muation Q61R and may be used to create syngeneic mouse melanoma models.

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SCC448
≥1X10⁶ cells/vial   
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      Replacement Information
      Description
      Catalogue NumberSCC448
      DescriptionOSUMMER.4 Mouse NRAS-Mutant Melanoma Cell Line
      Alternate Names
      • Mouse melanoma cell line
      Background InformationThe OSUMMER (Ohio State University and Moffitt Melanoma Exposed to Radiation) cell lines fulfill a previously unfilled gap in melanoma biology. These cell lines are syngeneic with C57BL/6 laboratory mice and have genetic profiles that are similar to those of human tumors, making them responsive to immunotherapy treatments. These NRAS-mutant cell lines can be used to discover and address potential immunotherapies in NRAS-mutant human melanomas which make up an estimated 15-30% of human melanomas. These cell lines also enable in vivo testing of immunotherapies with a mouse model.

      OSUMMER.4 NRAS mouse melanoma cell line was derived from a male TN mouse (homozygous for the LSL-NrasQ61R allele) exposed to UVA and UVB radiation. OSUMMER.4 can form tumors in vivo without Matrigel injection. WES (whole exome sequencing) revealed high SNV (Single Nucleotide variants) and low CNA (copy number alterations) burden, as well as the presence of UV signature mutations. OSUMMER.4 cells express the melanoma markers PMEL and MelanA and exhibit robust growth in culture.

      Source
      OSUMMER.4 was derived from melanoma induced in TN (Tyr::CreER) mice exposed to UVA or UVB radiation on postnatal day 3 (1).

      References
      1. Bowman, R. L., et al. (2021). UVB mutagenesis differs in Nras-and Braf-mutant mouse models of melanoma. Life Science Alliance, 4(9).
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationThe OSUMMER.4 melanoma cell line carries the NRAS muation Q61R and may be used to create syngeneic mouse melanoma models.
      Key Applications
      • Cell Culture
      • Cell Growth
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach vial contains ≥1X10⁶ viable cells.

      Cells are tested negative for infectious diseases by a Mouse Essential CLEAR Panel and have been found to be negative for all pathologies in this test.

      Cells are verified to be of mouse origin and negative for interspecies contamination from rat, human, Chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination Clear panel.

      Cells are negative for mycoplasma contamination.

      Cells are genotyped by STR analysis to verify the unique identity of the cell line.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsCells should be stored in liquid nitrogen. Cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      SCC448 04065268102022