530838 Sigma-AldrichNMD Inhibitor - CAS 307519-88-6 - Calbiochem
A cell-permeable inhibitor of nonsense-mediated mRNA decay (NMD) that enhances the stability of premature termination codon mutated p53 mRNA in N417 and HDQP-1 cells.
More>> A cell-permeable inhibitor of nonsense-mediated mRNA decay (NMD) that enhances the stability of premature termination codon mutated p53 mRNA in N417 and HDQP-1 cells. Less<<Synonymes: Ethyl 2-(((6,7-dimethyl-3-oxo-1,2,3,4-tetrahydro-2-quinoxalinyl)acetyl)amino)-4,5-dimethyl-3-thiophenecarboxylate, Nonsense-Mediated mRNA Decay Inhibitor 14, NMDI-14
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Tableau de caractéristiques principal
CAS # | Empirical Formula |
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307519-88-6 | C₂₁H₂₅N₃O₄S |
Prix & Disponibilité
Référence | Disponibilité | Conditionnement | Qté | Prix | Quantité | |
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5308380001 |
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Flacon en verre | 10 mg |
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Description | |
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Overview | A cell-permeable tetrahydroquinoxalinyl-thiophenecarboxylate compound that inhibits nonsense-mediated mRNA decay (NMD) in a dose-dependent manner and enhances the stability of premature termination codon (PTC) mutated p53 mRNA in N417 and HDQP-1 cells. However, it does not affect the stability of wild-type p53 in U2OS cells. Shown to dock reversibly within a SMG7 pocket and disrupt SMG7-UPF1 interaction and prevent their complex formation. Acts synergistically with G418 (Cat. No. 345810; 200 µg/ml) to increase p53 expression in p53 PTC mutant cells leading to increase mRNA levels of p21, Bax, and PUMA. Does not display any toxicity (> 50 µM) in multiple cell lines and has no effect on proliferation and protein synthesis in transformed and non-transformed cells. Please note that the molecular weight for this compound is batch-specific due to variable water content. |
Catalogue Number | 530838 |
Brand Family | Calbiochem® |
Synonyms | Ethyl 2-(((6,7-dimethyl-3-oxo-1,2,3,4-tetrahydro-2-quinoxalinyl)acetyl)amino)-4,5-dimethyl-3-thiophenecarboxylate, Nonsense-Mediated mRNA Decay Inhibitor 14, NMDI-14 |
References | |
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References | Martin, L., et al. 2014. Cancer Res. 74, 3104. |
Product Information | |
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CAS number | 307519-88-6 |
Form | Yellow powder |
Hill Formula | C₂₁H₂₅N₃O₄S |
Chemical formula | C₂₁H₂₅N₃O₄S |
Reversible | Y |
Quality Level | MQ100 |
Biological Information | |
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Primary Target | SMG7 |
Purity | ≥95% by HPLC |
Physicochemical Information | |
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Cell permeable | Y |
Packaging Information | |
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Packaged under inert gas | Packaged under inert gas |
Global Trade Item Number | |
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Référence | GTIN |
5308380001 | 04055977243000 |