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SCC297 N2A Psen1/Psen2 Double Knockout Mouse Neuroblastoma Cell Line

The N2A Psen1/2 cell line is a unique tool for the assessment of the contribution of PSEN mutations to AD pathology and widens the path to targeted therapies for AD and related neurodegenerative diseases.

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SCC297
≥1X10⁶ cells/vial l  
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      Replacement Information
      Description
      Catalogue NumberSCC297
      DescriptionN2A Psen1/Psen2 Double Knockout Mouse Neuroblastoma Cell Line
      OverviewEarly-onset Alzheimer s disease (AD), also termed autosomal dominant AD with early onset (ADAD), is a devastating neurodegenerative condition that is strongly linked to familial inheritance. The main cause of early-onset/familial AD is autosomal dominant
      Background InformationThe N2A Psen1/Psen2 cell line is a model for studies of presenilin function in the pathology of familial Alzheimer’s Disease. Early-onset Alzheimer’s disease (AD), also termed autosomal dominant AD with early onset (ADAD), is a devastating neurodegenerative disease that is strongly linked to familial inheritance. The main cause of early-onset/familial AD is autosomal dominant mutations in APP (amyloid precursor protein), PSEN1 (presenilin-1) or PSEN2 (presenilin-2).1 As catalytic subunits of γ-secretase complex, which cleaves APP to form amyloid beta peptides, Psen1 and Psen2 play significant roles in the formation of amyloid plaques. Variations in PSEN1/2 are frequently present in familial early-onset AD cases but their functional significance is unknown. Unraveling the functions of presenilin proteins is thus key to understanding the molecular pathology of AD.

      Source
      The N2A Psen1/2 cell line was derived via CRISPR/Cas9-editing of the parental N2A mouse neuroblastoma cell line.2 The N2A (Neuro2a) parental cell line was derived from neuroblastoma stem cells of a male strain A/J mouse.4

      References
      1. Holtzman DM, Morris JC. Goate AM. Sci Transl Med 2011; 3(77): 77sr1.
      2. Pimenova AA, Goate AM. Neurobio Dis 2020; 138:104785.
      3. Lee E-S, Jeong S-J, Kim Y-H, Jeon C-J. Acta Histochem Cytochem 2015; 48(6): 205-214.
      4. Olmsted JB, Carlson K, Klebe R, Ruddle F, Rosenbaum J. Proc Natl Acad Sci USA 1970; 65(1): 129-136.
      References
      Product Information
      Applications
      ApplicationThe N2A Psen1/2 cell line is a unique tool for the assessment of the contribution of PSEN mutations to AD pathology and widens the path to targeted therapies for AD and related neurodegenerative diseases.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach vial contains 1X10⁶ viable cells.
      Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
      Cells are verified to be of mouse origin and negative for inter-species contamination from rat, Chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
      Cells are negative for mycoplasma contamination.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsCells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial l
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      SCC297 04065266515640