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SCR108 Human Mesenchymal Stem Cell Kit - Derived from Bone-Marrow

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SCR108
1 kit containg >1 million viable cells and 500 mL of optimized media  
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      Key Applications
      Cult
      Description
      Catalogue NumberSCR108
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionHuman Mesenchymal Stem Cell Kit - Derived from Bone-Marrow
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationThis Human Mesenchymal Stem Cell Kit contains 1 million viable cells & 500ml of optimized media.
      Key Applications
      • Stem Cell Culture
      Application NotesMesenchymal stem cells, also known as marrow stromal cells (1), are defined as a self-renewing population of adherent, bone-marrow-derived multipotent progenitor cells with the capacity to differentiate into several mesenchymal cell lineages. In defined in vitro assays, mesenchymal stem cells have been shown to readily differentiate into lineage-specific cells that form bone, cartilage, fat, tendon, and muscle tissues (1, 2). Mesenchymal stem cells also provide support and maintenance for the other major stem cell population in the bone marrow, the hematopoietic stem cells (2).
      Mesenchymal stem cells (MSC’s) have historically been isolated based on the ability of these cells to form adherent cell layers in culture and the concomitant lack of adherence of other cells in the bone marrow stroma such as hematopoietic stem cells, adipocytes, and macrophage (1, 3). While this procedure results in enriched populations of mesenchymal stem cells, the resulting bone-marrow derived cell populations are, nonetheless, heterogeneous – comprised not only of mesenchymal stem cells, but also of committed lineage-restricted progenitors (1, 3). To aid researchers in the accurate identification and characterization of mesenchymal stem cells, Millipore provides cryopreserved Human Mesenchymal Stem Cells and optimized media in this kit. Millipore’s cryopreserved Human Mesenchymal Stem Cells are ready-to-use primary mesenchymal stem cells isolated from the iliac crest of normal human bone marrow (18-30 years old).
      Biological Information
      SourceHuman bone marrow
      Stem Cell Type
      • Mesenchymal Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceMillipore’s Human Mesenchymal Stem Cells are derived from human bone marrow and have been validated for high expression level of cell surface molecules that are present on mesenchymal stem cells: H-CAM (CD44), THY-1 (CD90) and STRO-1 and for their absence of hematopoietic cell surface markers, CD14 (present on leukocytes) and CD19 (present on B-lymphocytes). Millipore’s Human Mesenchymal Stem Cells are also negative for the endothelial marker, M-CAM (CD146). The cells have also been validated for their self-renewal and multi-lineage differentiation capacities (please refer to product manual figures for representative data). Cells display normal karyotype as assessed by G-banding of 20 metaphase cells and tested negative for mycoplasma. Each lot of Mesenchymal Stem Cell medium is tested for the optimal cell growth and proliferation of mesenchymal stem cells. Sterility Testing: Negative
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsHuman Mesenchymal Stem Cells (Part # SCC034) should be stored in liquid nitrogen.
      Mesenchymal Stem Cell Expansion Medium: (Catalog No. SCM015) should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month.
      Packaging Information
      Material Size1 kit containg >1 million viable cells and 500 mL of optimized media
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      SCR108 04053252005190

      Documentation

      Human Mesenchymal Stem Cell Kit - Derived from Bone-Marrow FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      Self-organizing tissue-engineered constructs in collagen hydrogels.
      Robert G Gourdie,Tereance A Myers,Alex McFadden,Yin-Xiong Li,Jay D Potts
      Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada  18  2011

      Afficher le résumé
      22214557 22214557

      Brochure

      Titre
      Human Stem Cell Systems

      Informations techniques

      Titre
      A Novel Serum-free and Xeno-free Differentiation Medium for Accelerated Osteogenic Differentiation of Human Mesenchymal Stem Cells
      LentiBrite™ Lentiviral Biosensors for Fluorescent Cellular Imaging: Analysis of Autophagosome Formation

      Produits & Applications associés

      Accessories

      Référence Description  
      SCM015 Mesenchymal Stem Cell Expansion Medium (1x) Prix & Disponibilité
      SCR067 Human Mesenchymal Stem Cell Characterization Kit Prix & Disponibilité

      Catégories

      Life Science Research > Cell Culture and Systems > Cell Lines > Stem Cell Lines