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331569 Ethidium Bromide Adsorber - CAS 69011-20-7 - Calbiochem

Developed specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels.

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331569
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CAS #
69011-20-7

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331569-1EA
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      Description
      OverviewDeveloped specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5 - 10 mg/L. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.
      Catalogue Number331569
      Brand Family Calbiochem®
      Application Data
      In order to begin flow, the two plug caps must be removed from the adaptors. These are tightly fit and may require some effort to remove them.
      References
      ReferencesMacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res. 48, 103.
      Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.
      Product Information
      CAS number69011-20-7
      ATP CompetitiveN
      FormGray-brown suspension
      ReversibleN
      Quality LevelMQ200
      Applications
      Biological Information
      Primary TargetRemoval of ethidium bromide (EtBr) from aqueous staining solutions and running buffers
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36

      Irritating to eyes.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Special InstructionsWhen the adsorber column is not in use, both ends should be closed off. The column should not be allowed to dry out, as air bubbles may form in the column, hindering flow rate.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      331569-1EA 04055977195750

      Documentation

      Ethidium Bromide Adsorber - CAS 69011-20-7 - Calbiochem FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Ethidium Bromide Adsorber - CAS 69011-20-7 - Calbiochem Certificats d'analyse

      TitreNuméro de lot
      331569

      Références bibliographiques

      Aperçu de la référence bibliographique
      MacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res. 48, 103.
      Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-December-2010 RFH
      Application Data
      In order to begin flow, the two plug caps must be removed from the adaptors. These are tightly fit and may require some effort to remove them.
      DescriptionDeveloped specifically for the safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions and running buffers used in nucleic acid separation gels. Typical concentrations in these solutions are 0.5-10 mg/l. EtBr is bound and concentrated by the adsorber column, allowing removal of the filtrate. Each column normally binds at least 300 mg of ethidium bromide from TAE or TBE buffers. Residual capacity is easily visualized because the EtBr appears as a dark red to black band on the column. As long as this band has not reached the bottom of the column bed, the concentration of dye is still below the column capacity.
      FormGray-brown suspension
      Recommended reaction conditions

      Ethidium bromide (EtBr) is the fluorescence dye most frequently used to stain nucleic acids in agarose and polyacrylamide gels. It intercalates primarily with double-stranded DNA to form an orange-red fluorescent complex under the influence of UV light. This interaction with nucleic acids makes ethidium bromide potentially mutagenic and is classified as toxic.

      Capacity

      The column capacity is at least 300 mg EtBr, corresponding to 30 L gel staining solution. The binding capacity of the cartridge is tested with a 10 mg/L EtBr solution of 1X TAE buffer; >99% of the dye was adsorbed up to the 300 mg limit as measured by fluorescence spectrometry.

      Flow Rate

      The flow rate of the adsorber column depends on the volume and height of the reservoir used for the staining solution and is a minimum of 200 ml/hr.

      Connecting the Adsorber Column

      The cartridge, as supplied, is sealed at both ends with small plastic caps (see figure 1). These caps should be removed prior to use and the cartridge connected to a reservoir containing the ethidium bromide solution to be adsorbed. A glass or plastic reservoir fitted with a stopcock or tubing clamp should be used to that the column can be switched on and off as required. To avoid any blocking of the frits with gel particles in the staining solutions, it is advisable to put some glass wool, filter paper, etc. in the connecting tube. If the flow rate of the adsorber column is distinctly lower than 1-2 drops per second, residual air bubbles must be removed by repeatedly short-squeezing the tubing above the cartridge. To guarantee a good flow rate throughout the lifespan of the cartridge, the formation of air bubbles in the system should be avoided, as these tend to hinder flow. The residual capacity of the column is easy to assess visually as the ethidium bromide moves through the column in the form of an increasingly dark red to black band. As long as this band has not reached the bottom of the column, the concentration of dye is still below the specified detection limit.

      Figure 1: Ethidium Bromide Adsorber

      In order to begin flow, the two plug caps must be removed from the adaptors. These are tightly fit and may require some effort to remove them.



      Disposal

      Once the capacity has been exhausted, the adsorber column should be disposed of according to local regulations.
      CAS number69011-20-7
      Storage +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsWhen the adsorber column is not in use, both ends should be closed off. The column should not be allowed to dry out, as air bubbles may form in the column, hindering flow rate.
      Toxicity Irritant
      ReferencesMacGregor, J.T., and Johnson, I.J. 1977. Mutat. Res. 48, 103.
      Waring, M. 1975. In: Antibiotics, Corcoran, J.W., and Hahn, F.E., Editors, Vol. 3, 141, Cold Spring Harbor, NY.