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SCT068 BioTracker DUAL dUTPase Live Cell reporter

Live cell probe for activity of cellular enzyme deoxyuridine triphosphatase
(dUTPase) involved in deactivation of damaged nucleotides. The probe acts as a luciferase luminescence
reporter of dUPTase.

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SCT068
1 mg  
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      Aperçu

      Replacement Information
      Description
      Catalogue NumberSCT068
      DescriptionBioTracker DUAL dUTPase Live Cell reporter
      OverviewNucleotide surveillance enzymes play important roles in
      human health, by monitoring damaged monomers in the nucleotide pool
      and deactivating them before they are incorporated into chromosomal
      DNA or disrupt nucleotide metabolism. Deamination of
      cytosine, leading to uracil in DNA and in the nucleotide pool, can be
      deleterious, causing DNA damage. The enzyme deoxyuridine triphosphatase
      (dUTPase) is the sole enzyme that cleanses the nucleotide pool of deaminated cytosine. Measuring the activity of this enzyme is important
      both in basic research and in clinical applications involving this
      pathway.

      The BioTracker DUAL dUTPase live cell probe is a luminescence probe that specifically reports on dUTPase activity in real time. The probe is a chimera of dUTP and ATP. dUTPase enzyme accepts the DUAL probe as an efficient substrate and upon hydrolysis releases ATP, which is detected sensitively by luciferase luminescence.

      Note: Luceferin and luciferase enzyme are needed to detect ATP released by hydrolysis, but are not included in this product.

      Spectral Properties
      Luminescence intensity.

      References
      Ji D et al. ATP-Linked Chimeric Nucleotide as a Specific Luminescence Reporter
      of Deoxyuridine Triphosphatase. Bioconjugate Chem. 2018, 29, 1614-1621.
      Alternate Names
      • Live cell imaging probe
      References
      Product Information
      Detection methodChemiluminescence
      PresentationLyophilized
      Applications
      ApplicationLive cell probe for activity of cellular enzyme deoxyuridine triphosphatase
      (dUTPase) involved in deactivation of damaged nucleotides. The probe acts as a luciferase luminescence
      reporter of dUPTase.
      Key Applications
      • Cell Based Assays
      • Activity Assay
      Application NotesLive cell fluorescent imaging
      Biological Information
      ConcentrationPlease refer to lot specific datasheet.
      Molecular Weight865.5 g/mol
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssurancePurity: ≥ 98% confirmed by LCMS. HNMR, PNMR and LC-MS confirms structure. Molar Mass: 865.5 g/mol
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore BioTracker DUAL dUTPase Live Cell Dye at -20°C, desiccate and protect from light
      Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      SCT068 04061841078104

      Documentation