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SCCAR10064-1VL Assay Ready LX-2 Human Hepatic Stellate Cell Line

LX-2 cells retain key features of human hepatic stellate cells including cytokine signaling, retinoid metabolism, and fibrogenesis. **SCCAR10064 cells are non-expandable and packaged at high density to enable readiness for hepatic fibrosis and other relevant assays without propagation or passage.

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      Replacement Information
      Description
      Catalogue NumberSCCAR10064-1VL
      DescriptionAssay Ready LX-2 Human Hepatic Stellate Cell Line
      OverviewHepatic stellate cells are a major cell type responsible for liver fibrosis following their activation into fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. There is an increasing need for renewable cell culture models that faithfully recapitulate their in vivo phenotype, particularly for human studies.

      LX-2 was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early passaged cells in low serum media conditions.

      Immortalized LX-2 was established by Xu et al to overcome issues of culture variability and to provide a stable and unlimited source of human hepatic stellate cells that are homogeneous. These cell lines have been extensively characterized and retain key features of cytokine signaling, neuronal gene expression, retinoid metabolism, and fibrogenesis, making them highly suitable for culture based studies of human hepatic fibrosis.
      Alternate Names
      • assay ready hepatocytes
      • assay ready hepatic stellate cells
      • hepatic stellate cell line
      • hepatocyte cell line
      • hepatic fibrosis cell line
      Background InformationThis product contains cells sold under a consumable label license and which may not be propagated. For the equivalent expandable, bankable LX-2 cell line, please see SCC064.


      Hepatic stellate cells are an essential cell phenotype responsible for liver fibrosis following their activation to fibrogenic myofibroblast-like cells in diseases such as chronic alcoholism, hepatitis B and C, fatty liver disease, obesity and diabetes. There is an increasing need for renewable cell culture models that faithfully recapitulate their in vivo phenotype, particularly for the development of therapeutics.

      The LX-2 cell line was generated by immmortalization of primary human hepatic stellate cells with the SV40 large T antigen followed by selective culture of early-passage cells in low serum media conditions.
      References
      Product Information
      Components
      • 1 vial LX-2 Human Hepatic Stellate Cells (Catalog No. SCCAR10064-1VL). Store in liquid nitrogen.
      Quality LevelMQ100
      Applications
      ApplicationLX-2 cells retain key features of human hepatic stellate cells including cytokine signaling, retinoid metabolism, and fibrogenesis. **SCCAR10064 cells are non-expandable and packaged at high density to enable readiness for hepatic fibrosis and other relevant assays without propagation or passage.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale as a consumable. It may not be propagated, passaged, or expanded and is subject to the terms of the “Assay Ready Cells Research Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
      Biological Information
      Cell Line Type
      • Hepatocyte Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance
      • Cells are tested by PCR and are negative for Hepatitis A, B, C and HIV-1 & 2 viruses.
      • Cells are negatrive for mycoplasma contamination.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Storage ConditionsLX-2 cells should be stored in liquid nitrogen.
      Packaging Information
      Material Size1 vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      SCCAR10064-1VL 04065270631497