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558300 Aquatic Block™ Reagent, Salmon Plasma

Aquatic Block™ Reagent, Salmon Plasma : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
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558300
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558300-500ML
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      Description
      OverviewA ready-to-use blocking reagent for Western blotting and immunoassays. Contains salmon plasma proteins that do not interact with mammalian antibodies, thus offering a reduced background.
      Catalogue Number558300
      Brand Family Calbiochem®
      References
      Product Information
      FormLiquid
      FormulationIn PBS, 0.09% NaN₃, pH 7.4.
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      558300-500ML 04055977193039

      Documentation

      Aquatic Block™ Reagent, Salmon Plasma FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Aquatic Block™ Reagent, Salmon Plasma Certificats d'analyse

      TitreNuméro de lot
      558300
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-August-2022 JSW
      DescriptionA ready-to-use blocking reagent containing non-mammalian proteins that do not interact with mammalian antibodies.
      FormLiquid
      FormulationIn PBS, 0.09% NaN₃, pH 7.4.
      Recommended reaction conditions
      Use at 1X concentration or further dilution depending on specific use. BioAssay results using Aquatic Block™ Reagent as a blocking reagent Comparison of Aquatic Block™ Reagent to standard blockers: 3% BSA, 0.2% Non Fat Dry Milk, and 10% Fetal Bovine Serum. All assays were performed following standard protocols, except the blocking reagent was added prior to the addition of the antigen and/or antibody. All measurements reported as average Absorbance. The blank well was subtracted from the actual readings.

      Table 1: Sample Wells

      Medium Binding: All the blocking reagents exhibit excellent blocking ability on the surface-BSA being the best performer by a slight margin. Note: blocking mechanism for this surface is hydrophobic. High Binding: All the blocking reagents exhibit excellent blocking ability on this surface-FBS being the best performer by a very small margin. BSA, the only single protein type blocker, exhibited the least effective blocking (still excellent) as compared to the other blockers. Since a high binding surface is a combination of hydrophobic and ionic binding mechanisms, a blocker comprised of several proteins typically performs better. Glutaraldehyde Activated Amine (Polystyrene): BSA is a poor blocker for this surface. Typically a covalent surface such as this one (combination of Hydrophobic, ionic and covalent binding mechanisms) requires a multicomponent blocking reagent- such as a normal serum. The best blocker for this surface was fish serum, followed by fetal bovine serum and non-fat dry milk. N-oxysuccinimide: This covalent surface is comprised of NOS groups and hydrophobic spacer arms. FBS was the best performer, fish serum and NFDM also performed adequately. None of these blocking reagents can be promoted as universal blockers, since they are surface dependent.

      Table 2: Ranking per Surface Type: Best to Worst

      Note: There are only very minor differences in many cases between the blockers and one can not call one better than the other. It is obvious that BSA is the poorest blocker, although it is highly promoted as a "superior" blocking reagent. Fish serum blocked all of the surfaces very well.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Toxicity Standard Handling