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OP03L Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)

This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic).

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OP03L
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Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
H, Mk, M, Rb, RMMonoclonal Antibody

Prix & Disponibilité

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OP03L-100UG
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      Description
      OverviewRecognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated p53.
      Catalogue NumberOP03L
      Brand Family Calbiochem®
      References
      ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Legros, Y., et al. 1994. Oncogene 9, 2071.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991. Cancer Res. 51 6304.
      Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709.
      Harlow, E., et al. 1981. J. Virol. 39 861.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Negative controlNormal skin tissue or SK-OV-3 cells
      Positive controlBreast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Wang, H., et al. 1991. Cell Growth and Diff. 2, 645. Immunofluorescence, Frozen Sections Davidoff, A.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 5006. Immunoprecipitation Lehman, T.A., et al. 1991. Cancer Res. 51, 4090. Harlow, E., et al. 1981. J. Virol. 39, 861. Original Clone Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709. Harlow, E., et al. 1981. J. Virol. 39 861. Epitope Identification Legros, Y., et al. 1994. Oncogene 9, 2071. Waterman, M.J.F., et al. 1998. Nature Genetics 19, 175. (within amino acids 376-378) Cross Reactivity/Phosphophorylated p53/Acetylated p53 Li, M., et al. 2002. J. Biol. Chem. 227, 50607. Delphin, C. and Baudier, J. 1994. J. Biol. Chem. 269, 29579.
      Key Applications Flow Cytometry
      Frozen Sections
      Gel Shift
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesFrozen Sections (10 µg/ml, see application references)
      Gel Shift (see comments)
      Immunoblotting (10 µg/ml, see application references)
      Immunofluorescence (1-20 µg/ml, see application references)
      Flow Cytometry (1-20 µg/ml)
      Immunoprecipitation (1 µg per sample, see application references)
      Application CommentsOriginally called clone L21. Does not recognize phosphorylated or acetylated p53. For a gel shift assay, resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then perform immunoblotting using Cat. No. PC35 and chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpartially purified mouse p53
      ImmunogenMouse
      Epitopewithin amino acids 376-378 of human p53
      ClonePAb421
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Monkey
      • Mouse
      • Rabbit
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide; freezing of aliquots is best for long-term storage of reconstituted product, but repetitive freezing and thawing should be avoided.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      OP03L-100UG 04055977209594

      Documentation

      Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) Certificats d'analyse

      TitreNuméro de lot
      OP03L

      Références bibliographiques

      Aperçu de la référence bibliographique
      El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Legros, Y., et al. 1994. Oncogene 9, 2071.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991. Cancer Res. 51 6304.
      Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709.
      Harlow, E., et al. 1981. J. Virol. 39 861.

      Brochure

      Titre
      Interactive Pathway Calendar
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-July-2008 JSW
      ApplicationFrozen Sections (10 µg/ml, see application references)
      Gel Shift (see comments)
      Immunoblotting (10 µg/ml, see application references)
      Immunofluorescence (1-20 µg/ml, see application references)
      Flow Cytometry (1-20 µg/ml)
      Immunoprecipitation (1 µg per sample, see application references)
      DescriptionPurified mouse monoclonal antibody, originally called clone L21, generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~53 kDa wild-type and mutant forms of p53.
      BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
      HostMouse
      Immunogen speciesMouse
      Immunogenpartially purified mouse p53
      Epitopewithin amino acids 376-378 of human p53
      ClonePAb421
      IsotypeIgG2a
      Specieshuman, monkey, mouse, rabbit, rat
      Positive controlBreast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
      Negative controlNormal skin tissue or SK-OV-3 cells
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsOriginally called clone L21. Does not recognize phosphorylated or acetylated p53. For a gel shift assay, resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then perform immunoblotting using Cat. No. PC35 and chemiluminescent detection. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Store at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide; freezing of aliquots is best for long-term storage of reconstituted product, but repetitive freezing and thawing should be avoided.
      Toxicity Standard Handling
      ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
      Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
      Legros, Y., et al. 1994. Oncogene 9, 2071.
      Barak, Y., et al. 1993. EMBO J. 12, 461.
      Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
      Kastan, M.B., et al. 1992. Cell 71, 587.
      Lane, D.P. 1992. Nature 358, 15.
      Kastan, M.B., et al. 1991. Cancer Res. 51 6304.
      Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709.
      Harlow, E., et al. 1981. J. Virol. 39 861.
      Application referencesImmunoblotting Wang, H., et al. 1991. Cell Growth and Diff. 2, 645. Immunofluorescence, Frozen Sections Davidoff, A.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 5006. Immunoprecipitation Lehman, T.A., et al. 1991. Cancer Res. 51, 4090. Harlow, E., et al. 1981. J. Virol. 39, 861. Original Clone Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709. Harlow, E., et al. 1981. J. Virol. 39 861. Epitope Identification Legros, Y., et al. 1994. Oncogene 9, 2071. Waterman, M.J.F., et al. 1998. Nature Genetics 19, 175. (within amino acids 376-378) Cross Reactivity/Phosphophorylated p53/Acetylated p53 Li, M., et al. 2002. J. Biol. Chem. 227, 50607. Delphin, C. and Baudier, J. 1994. J. Biol. Chem. 269, 29579.

      Produits & Applications associés

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      OP03 Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) Prix & Disponibilité

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      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies