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ABE472 Anti-WAC Antibody, NT

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ABE472
100 µg  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IP, ChIPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE472
      DescriptionAnti-WAC Antibody, NT
      Alternate Names
      • WW domain-containing adapter protein with coiled-coil
      Background InformationWAC (WW domain-containing adapter protein with coiled-coil) functions as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Wac interacts with the RNA polymerase II transcriptional machinery through its WW domain and with RNF20-RNF40 via its coiled-coil region. It has been shown to regulate H2BK120ub1, gene transcription, cell-cycle checkpoint activation in response to DNA damage, positively regulate amino acid starvation-induced autophagy and negatively regulate the ubiquitin proteasome pathway. In nuclear speckles it has been observed to colocalize with pre-mRNA processing complexes. This antibody recognizes the N-terminus of the WAC protein.
      References
      Product Information
      FormatAffinity Purified
      Control
      • K562 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-WAC, N-terminus detects levels of WAC proteins & has been published & validated for use in WB, IP & ChIP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected WAC in 10 µg of HEK293 cell lysate.

      Immunoprecipitation Analysis: A representative lot from an independent laboratory detected WAC, N-terminus in 293T cell lysates stably transfected with SFB-RNF20 and in HCT116 cell lysates transfected with siRNA negative control (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).

      Chromatin Immunoprecipitation: A representative lot from an independent laboratory detected WAC, N-terminus in HCT116 cell lysates transfected with siRNA negative control, but demonstrated a loss of signal in HCT166 cell lysates transfected with siRNF20 (Zhang, F. and Yu, X. (2011). Mol Cell. 41(4):384-397.).
      Biological Information
      ImmunogenRecombinant protein corresponding to human WAC.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • WAC
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~80 kDa observed. Multiple isoforms are known to exist at ~70 kDa, ~65 kDa, ~60 kDa, and ~47 kDa. Uncharacterized bands may be observed at ~52 kDa, ~75 kDa, and ~270 kDa in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected WAC, N-terminus in 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      ABE472 04053252858222

      Documentation

      Anti-WAC Antibody, NT FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-WAC Antibody, NT Certificats d'analyse

      TitreNuméro de lot
      Anti-WAC, N-terminus - 3220350 3220350
      Anti-WAC, N-terminus - 3310132 3310132
      Anti-WAC, N-terminus - 3698192 3698192
      Anti-WAC, N-terminus - 3873511 3873511
      Anti-WAC, N-terminus - Q2141869 Q2141869
      Anti-WAC, N-terminus -2539270 2539270

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      WAC, a functional partner of RNF20/40, regulates histone H2B ubiquitination and gene transcription.
      Zhang, Feng and Yu, Xiaochun
      Mol. Cell, 41: 384-97 (2011)  2010

      Afficher le résumé
      21329877 21329877

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      Référence Description  
      ABE471 Anti-WAC Antibody, CT Prix & Disponibilité

      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies