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MAB1096-25UG Anti-Tumor Necrosis Factor-alpha,cl 195

This Anti-Tumor Necrosis Factor-α Antibody, clone 195 is validated for use in IC, IH, NEUT, WB for the detection of Tumor Necrosis Factor-α.

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MAB1096-25UG
25 μg  
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      Description
      Catalogue NumberMAB1096-25UG
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tumor Necrosis Factor-alpha,cl 195
      Alternate Names
      • TNF-alpha
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid in 0.01M PBS pH 7.1, 0.15M NaCl containing no preservatives. Sterile filtered.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Tumor Necrosis Factor-α Antibody, clone 195 is validated for use in IC, IH, NEUT, WB for the detection of Tumor Necrosis Factor-α.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Neutralizing
      • Western Blotting
      Application NotesWestern Blot

      Immunocytochemistry

      Immunohistochemistry

      Neutralization: 50 ng of TNF-alpha antibody is required for the neutralization of 1 U TNF-alpha. The TNF-alpha-preparation which is used has a specific activity of 2 x 10(7) U/mg protein [cell lytic assay with L929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D].

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenHuman, recombinant tumor necrosis factor-alpha produced in E. coli.
      Clone195
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThe antibody reacts with human, natural and recombinant TNF-alpha. lt does not react with human TNF-beta (lymphotoxin). lt does not react with TNF-alpha from mouse, rat, canine, porcine, rhesus-monkey or baboon.

      SPECIES REACTIVITIES:

      Not mouse, rat, canine, porcine, baboon or rhesus-monkey.
      IsotypeIgG3
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
      Gene Symbol
      • TNF
      • OTTHUMP00000037669
      • TNFA
      • TNFSF2
      • cachectin
      • TNF-a
      • Cachectin
      • DIF
      • TNF-alpha
      Non-Reactive Species
      • Canine
      • Mouse
      • Monkey
      • Pig
      • Rat
      • Baboon
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01375 # Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
      SIZE: 233 amino acids; 25644 Da
      SUBUNIT: Homotrimer.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein. & Tumor necrosis factor, soluble form: Secreted.
      PTM: The soluble form derives from the membrane form by proteolytic processing. & The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
      DISEASE: SwissProt: P01375 # Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition. & Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis. & Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.
      SIMILARITY: SwissProt: P01375 ## Belongs to the tumor necrosis factor family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceTested
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Freeze only once.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MAB1096-25UG 04054839342646

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