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ABT256 Anti-Telo-Collagen Type I, A1/COL1A1

Anti-Telo-Collagen Type I Antibody, A1/COL1A1 is an antibody against Telo-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.

Anti-Telo-Collagen Type I, A1/COL1A1 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: COL1A1 UniProt Number: P02452
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ABT256
100 µL  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, ShWB, FC, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT256
      DescriptionAnti-Telo-Collagen Type I, A1/COL1A1
      Alternate Names
      • Collagen alpha-1(I) chain
      • Alpha-1 type I collagen
      Background InformationCollagen alpha-1(I) chain (UniProt P02452; also known as Alpha-1 type I collagen) is encoded by the COL1A1 gene (Gene ID 1277) in human. Collagen is the major component of the extracellular matrix (ECM) and form the fibrils of tendons, ligaments, and bones. Type I collagen consists of two alpha I chains and one alpha 2 chain. Alpha-1 type I collagen is initially produced as an 1464-amino acid prepro-form with a signal peptide sequence (a.a. 1-22) and two propeptide sequences (a.a. 23-161 and a.a. 1219 –1464), the removal of which yields the mature alpha-1(I) chain. The mature alpha-1(I) chain is composed mostly of a large triple-helical region (a.a. 179-1192) sandwiched between two nonhelical segments known as the N-terminal telopeptide (a.a. 162-178; numbering based on the prepro-form) and the C-terminal telopeptide (a.a. 1193-1218; numbering based on the prepro-form). Collagen can be extracted from tissue via either enzymatic or non-enzymatic means. Collagen extracted using the proteolytic enzyme pepsin corresponds to the large triple-helical region, referred to as atelocollagen because both the N- and C-terminal telopeptides have been cleaved off by pepsin. On the other hand, collagen preparations obtained with non-enzymatic means (e.g. by acid extraction) have the intact telopeptides at both ends.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Telo-Collagen Type I Antibody, A1/COL1A1 is an antibody against Telo-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung and skin tissue lysate.
      Flow Cytometry Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung tissue.
      Immunohistochemistry Analysis: A representative lot detected Telo-Collagen Type I, A1/COL1A1 in mouse lung tissue.
      Biological Information
      ImmunogenLinear peptide corresponding to sequence derived from human Collagen Type I, A1/COL1A1 C-terminal telopeptide region.
      EpitopeC-terminal telopeptide region
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityCat. No. ABT256 Anti-Telo-Collagen Type I, A1/COL1A1 was raised against a sequence derived from the C-terminal telopeptide (not the N-terminal telopeptide) region. This polyclonal antibody will detect prepro-, pro-, and mature collagen alpha-1(I) chain, but not atelocollagen prepared with proteolytic enzymes due to the absence of the C-terminal telopeptide sequence.
      Species Reactivity
      • Human
      • Mouse
      • Sheep
      Species Reactivity NoteHuman, Mouse, Sheep. Predicted to react with a broad range of species based on high sequence homology, including mammalian, avian, and amphibian species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • COL1A1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~180 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse liver tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Telo-Collagen Type I, A1/COL1A1 in 1 µg of mouse liver tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      ABT256 04055977136654

      Documentation

      Anti-Telo-Collagen Type I, A1/COL1A1 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Telo-Collagen Type I, A1/COL1A1 Certificats d'analyse

      TitreNuméro de lot
      Anti-Telo-Collagen Type I, A1/COL1A1 - 3245100 3245100
      Anti-Telo-Collagen Type I, A1/COL1A1 - 3426902 3426902
      Anti-Telo-Collagen Type I, A1/COL1A1 - 3436193 3436193
      Anti-Telo-Collagen Type I, A1/COL1A1 - 4059976 4059976
      Anti-Telo-Collagen Type I, A1/COL1A1 -2884269 2884269
      Anti-Telo-Collagen Type I, A1/COL1A1 -Q2564057 Q2564057

      Informations techniques

      Titre
      White Paper: Further considerations of antibody validation and usage.

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      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies