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MABN2402-100UG Anti-Tau Antibody, clone 10C5

This mouse monoclonal Anti-Tau, clone 10C5, Cat. No. MABN2402 is tested for use in Western Blotting, Immunohistochemistry, and ELISA, for the detection of Tau.

NouveauMABN2402-100UG
100 μg  
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      Replacement Information
      Description
      Catalogue NumberMABN2402-100UG
      DescriptionAnti-Tau Antibody, clone 10C5
      Alternate Names
      • Neurofibrillary tangle protein
      • Paired helical filament-tau (PHF-tau)
      • MAPTL
      • MTBT1
      • TAU
      • PR-1
      Background InformationMicrotubule-associated protein tau (UniProt: P10636; also known as Neurofibrillary tangle protein Paired helical filament-tau (PHF-tau)) is encoded by the MAPT (also known as MAPTL, MTBT1, TAU) gene (Gene ID: 4137) in human. Tau is a highly abundant protein found predominantly in the nervous system. Its primary role is to stabilize and support microtubule assembly, which are essential for maintaining the structure and function of nerve cells. It is present throughout the brain, particularly in regions involved in memory and cognition. MAPT gene consists of multiple exons that undergo alternative splicing, resulting in the generation of different isoforms that differ in the number and arrangement of repeats in the microtubule-binding domain. The expression of the MAPT transcripts varies and depends on the stage of neuronal maturation and neuron type. Aberrant aggregation and accumulation of tau protein have been linked to several neurodegenerative diseases collectively known as tauopathies. The most common tauopathy is Alzheimer's disease, where hyperphosphorylated tau protein forms neurofibrillary tangles inside neurons. Other tauopathies include frontotemporal dementia, progressive supranuclear palsy, and cortico-basal degeneration. These diseases are characterized by cognitive decline, motor disturbances, and memory loss.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationThis mouse monoclonal Anti-Tau, clone 10C5, Cat. No. MABN2402 is tested for use in Western Blotting, Immunohistochemistry, and ELISA, for the detection of Tau.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • ELISA
      Application NotesTested Applications

      Enzyme Immunoassay (ELISA): Serial dilutions from a representative lot detected Tau monomers or Tau aggregates induced with arachidonic acid in ELISA (Courtesy of Nicholas Kanaan, Ph.D., Michigan State University, USA).

      Western Blotting Analysis: A representative lot detected recombinant, human Tau isoform 2N4R monomers and aggregates, 2N3R monomers and aggregates, and mouse Tau monomers. (Courtesy of Nicholas Kanaan, Ph.D., Michigan State University, USA).

      Immunohistochemistry Applications: A representative lot detected Tau in Alzheimer's diseased (AD) brain (superior temporal cortex, STG) tissue sections. Human disease sections had to be treated with phosphatase (dephosphorylates tau) to obtain reactivity suggesting tau in human AD is phosphorylated at its epitope. (Courtesy of Nicholas Kanaan, Ph.D., Michigan State University, USA).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenHis-tagged, recombinant, human Tau isoform 1N3R aggregates produced with arachidonic acid.
      EpitopeN-terminal half
      Clone10C5
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 10C5 is a mouse monoclonal antibody that detects Tau. It targets an epitope within 52 amino acids in the proline-rich region (PR-1) of human Tau.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MAPT
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight ~57 kDa observed. 78.93 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with His-tagged, Human recombinant Tau, isoform 1N4R.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected His-tagged, Human, recombinant Tau isoform 1N4R.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABN2402-100UG 04065271175082