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PC177L Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb

This Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Serotonin (5-HT) Transporter (602-622).

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PC177L
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Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
M, RRbPolyclonal Antibody

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PC177L-100UL
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      Description
      OverviewRecognizes the serotonin (5-HT) transporter in hypothalamus raphe nuclei and spinal cord.
      Catalogue NumberPC177L
      Brand Family Calbiochem®
      Application Data
      Detection of rat serotonin (5-HT) transporter by immunohistochemistry. Sample: Rat raphe nuclei, hypthalamus, cortex, and spinal cord. Primary antibody: Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb (Cat. No. PC177L) (1:1000). Detection: fluorescence.
      References
      ReferencesZhou, F.C., et al. 1996. Mol. Brain Res. 43, 267.
      Blakely, R.D., et al. 1994. J. Exp. Biol. 196, 263.
      Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.
      Product Information
      FormLyophilized
      FormulationUndiluted serum.
      Positive controlRat naphe nuclei, hypothalamus, cortex, or spinal cord
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesFrozen Sections, Mouse Cross-Reactivity Salichon N., et al. 2001. J. Neuerosci. 21, 884.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesFrozen Sections (1:10,000-1:15,000; see application references)
      Immunoblotting (see comments)
      Immunofluorescence (1:200-1:400)
      Application CommentsAntibody specificity was examined in the rat cortex, hypothalamus, raphe nuclei, and spinal cord. Staining is completely eliminated by pretreatment of antibody with Serotonin (5-HT) Transporter Control Peptide (Cat. No. PP87) at a concentration of 5 µg/ml. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. The specificity of this antibody was also determined by immunoblotting analysis using rat brain extracts of the cortex, hypothalamus, midbrain, and hindbrain, which specifically detected a single band. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 602-622 of rat 5-HT transporter
      ImmunogenRat
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl of sterile distilled H₂O. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Further dilute with sterile PBS or Tris buffer to dilutions no higher than 1:10.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      PC177L-100UL 04055977209525

      Documentation

      Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb Certificats d'analyse

      TitreNuméro de lot
      PC177L

      Références bibliographiques

      Aperçu de la référence bibliographique
      Zhou, F.C., et al. 1996. Mol. Brain Res. 43, 267.
      Blakely, R.D., et al. 1994. J. Exp. Biol. 196, 263.
      Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-June-2008 JSW
      ApplicationFrozen Sections (1:10,000-1:15,000; see application references)
      Immunoblotting (see comments)
      Immunofluorescence (1:200-1:400)
      Application Data
      Detection of rat serotonin (5-HT) transporter by immunohistochemistry. Sample: Rat raphe nuclei, hypthalamus, cortex, and spinal cord. Primary antibody: Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb (Cat. No. PC177L) (1:1000). Detection: fluorescence.
      DescriptionRabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the serotonin (5-HT) transporter protein.
      BackgroundThe serotonin (5-hydroxytryptamine; 5-HT) transporter (5-HTT) plays an important role in the re-uptake of serotonin from the nerve terminal to ultimately terminate the serotonin signal. The 5-HTT plays a critical role in the regulation of serotonin neurotransmission, which has been linked to mood and personality disorders, anxiety, eating disorders, and insomnia. The predicted 12 transmembrane serotonin transporter contains potential sites for phosphorylation and glycosylation. Because serotonin is involved in such a variety of biological processes, the 5-HTT has been a target for the development of antidepressants and psychostimulants. Blocking serotonin uptake at the synapse has been achieved using structurally related chemicals called Selective Serotonin Re-uptake Inhibitors (SSRI) which alleviate disorders such as depression and alcoholism. The 5-HTT is localized to the plasma membrane of neurons and glial cells, as well as blood platelets, placenta, and endothelial cells.
      HostRabbit
      Immunogen speciesRat
      Immunogena synthetic peptide corresponding to amino acids 602-622 of rat 5-HT transporter
      IsotypeIgG
      Speciesmouse, rat
      Positive controlRat naphe nuclei, hypothalamus, cortex, or spinal cord
      FormLyophilized
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsAntibody specificity was examined in the rat cortex, hypothalamus, raphe nuclei, and spinal cord. Staining is completely eliminated by pretreatment of antibody with Serotonin (5-HT) Transporter Control Peptide (Cat. No. PP87) at a concentration of 5 µg/ml. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. The specificity of this antibody was also determined by immunoblotting analysis using rat brain extracts of the cortex, hypothalamus, midbrain, and hindbrain, which specifically detected a single band. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl of sterile distilled H₂O. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Further dilute with sterile PBS or Tris buffer to dilutions no higher than 1:10.
      Toxicity Standard Handling
      ReferencesZhou, F.C., et al. 1996. Mol. Brain Res. 43, 267.
      Blakely, R.D., et al. 1994. J. Exp. Biol. 196, 263.
      Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.
      Application referencesFrozen Sections, Mouse Cross-Reactivity Salichon N., et al. 2001. J. Neuerosci. 21, 884.