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ABN420 Anti-RalDH2 (ALDH1A2)

Anti-RalDH2 (ALDH1A2) Antibody, Cat. No. ABN420, is a highly specific rabbit polyclonal antibody that targets RalDH2 (ALDH1A2) and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-RalDH2 (ALDH1A2) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Neuroscience Gene Symbol: Aldh1a2, Aldh1a7, Raldh2 UniProt Number: Q62148
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ABN420
100 μg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIF, IH(P), WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN420
      DescriptionAnti-RalDH2 (ALDH1A2)
      Alternate Names
      • Retinal dehydrogenase 2
      • Aldehyde dehydrogenase family 1 member A2
      • RALDH 2
      • RalDH2
      • RALDH(II)
      • Retinaldehyde-specific dehydrogenase type 2
      Background InformationRetinal dehydrogenase 2 (EC 1.2.1.36; UniProt Q62148; also known as Aldehyde dehydrogenase family 1 member A2, RALDH 2, RalDH2, RALDH(II), Retinaldehyde-specific dehydrogenase type 2) is encoded by the Aldh1a2 (also known as Aldh1a7, Raldh2) gene (Gene ID 19378) in murine species. It can recognize and bind both free retinal and cellular retinol-binding protein-bound retinal and oxidize it to retinoate. The enzyme is known to be active in embryos, where retinoic acid play a crucial role in early development. RALDH2-null mice do not live beyond E10.5. The three retinaldehyde dehydrogenases, RALDH1-3 or ALDH1 alpha 1-3, catalyze the irreversible oxidation of retinaldehyde to form atRA. While retinaldehyde is formed via the reversible oxidation of retinol catalyzed by retinol dehydrogenases (RDHs). The three RALDH enzymes cooperatively mediate atRA signaling during eye development through tightly regulated spatiotemporal expression patterns. In the postnatal human eye, RALDH catalytic activity can be detected in the choroid, retinal pigment epithelium (RPE), and retina. RALDH2 is most abundant in the choroid and RPE, moderate abundance in the retina, and in relatively low abundance in sclera. RALDH1 is most abundant in the choroid, in moderate abundance in the sclera, and substantially reduced in the retina and RPE. RALDH3 is most abundantly expressed during embryonic eye development, but not detectable in adult ocular fundal tissues.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-RalDH2 (ALDH1A2) Antibody, Cat. No. ABN420, is a highly specific rabbit polyclonal antibody that targets RalDH2 (ALDH1A2) and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected RalDH2 in rat embryo tissue section.

      Immunofluorescence Analysis: A 1:300 dilution from a representative lot immunostained the developing eye in paraffin-embedded embryonic E15 Sprague Dawley (SD) rat head sections by fluorescent immunohistochemistry (Courtesy of Anna Ashton, University of Aberdeen).

      Western Blotting Analysis: A 1:3,000 dilution from a representative lot detected RalDH2 in 50 µg of new born (postnatal P0) Sprague Dawley (SD) rat whole eye tissue lysate, but not in adult SD rat brain lateral cortex and pons tissue lysates (Courtesy of Anna Ashton, University of Aberdeen).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to an N-terminal region sequence of mouse RalDH2.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody targets an N-terminal region sequence of human/mouse/rat RalDH2 reported by UniProt (O94788/Q62148/Q63639).
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Aldh1a2
      • Aldh1a7
      • Raldh2
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~57 kDa observed. 56.72/56.63/56.64 kDa (human/mouse/rat) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in mouse retina tissue section.

      Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected RalDH2 in mouse retina tissue section.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      ABN420 04054839114557