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16-204 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate

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16-204
100 µg  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AWBMBiotinMonoclonal Antibody
      Description
      Catalogue Number16-204
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate
      OverviewIncluded Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate

      Product Description: Produced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
      Background InformationProduced from CHO cells expressing the 4G10 antibody heavy and light chain cDNAs. Heavy chain C-terminus has a hexa-histidine tag for purification and immobilization via nickel affinity matrices. Cross-linked to biotin. Patent pending.
      Product Information
      FormatBiotin
      Control
      • A431 carcinoma cells.
        Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting.
      PresentationBiotin-conjugated recombinant mouse IgG2bκ in PBS containing 0.05% sodium azide. Liquid at 2-8°C.
      Quality LevelMQ100
      Applications
      ApplicationDetect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPhosphotyramine-KLH
      EpitopeN/A
      Clone4G10®
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityTyrosine-phosphorylated proteins from all species.
      IsotypeIgG2bκ
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Modifications
      • Phosphorylation
      Purification MethodProtein G Purified
      Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot to detect tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected tyrosine phosphorylated proteins in a RIPA lysate of EGF-stimulated A431 carcinoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 9 months at 2-8ºC from date of receipt.
      For maximum recovery of product, centrifuge the vial prior to removing the cap. DO NOT FREEZE!
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Référence GTIN
      16-204 04053252366734