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MAB5240 Anti-Phosphacan Antibody

Detect Phosphacan using this Anti-Phosphacan Antibody validated for use in ELISA, WB, IH(P).

Anti-Phosphacan Antibody : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Neuroscience Research Sub-Category: Growth Cones & Axon Guidance Gene Symbol: PTPRZ1, PTPRZ, PTPZ UniProt Number: Q62656
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MAB5240
1 mL  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      ChELISA, WB, IH(P)MCulture SupernatantMonoclonal Antibody
      Description
      Catalogue NumberMAB5240
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Phosphacan Antibody
      Background InformationPhosphacan is a soluble nervous tissue-specific proteoglycan that is thought to regulate axonal outgrowth. It is synthesized by glia and binds to neurons and to the neural cell adhesion molecules tenascin, N-CAM or NG-CAM but not to laminin and fibronectin. Phosphacan acts as a potent inhibitor of cell adhesion and neurite outgrowth.
      References
      Product Information
      FormatCulture Supernatant
      PresentationTissue culture supernatant. Liquid. Contains no preservative.
      Quality LevelMQ100
      Applications
      ApplicationDetect Phosphacan using this Anti-Phosphacan Antibody validated for use in ELISA, WB, IH(P).
      Key Applications
      • ELISA
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot: 1:1,000

      Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.

      Immunohistochemistry: 1:20 Works on cold methanol or paraformaldehyde fixed tissues. Works on paraffin embedded tissues. Chondrotinase treatment of tissue consists of treating mounted tissue section with a solution of PBS plus 0.5U/mL of Chondroitinase ABC for 30 minutes at room temperature prior to blocking and antibody staining.

      Immunocytochemistry: not tested

      ELISA: Excellent for core and monomer protein.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenMixture of chicken chondroitin sulfate proteoglycans.
      HostMouse
      SpecificityPhosphacan (Receptor Tyrosine Phosphatase Beta).
      IsotypeIgM
      Species Reactivity
      • Chicken
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • PTPRZ1
      • PTPRZ
      • PTPZ
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size1 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MAB5240 04053252398704

      Documentation

      Anti-Phosphacan Antibody FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Phosphacan Antibody Certificats d'analyse

      TitreNuméro de lot
      MOUSE ANTI-PHOSPHACAN (CHICKEN) (RECEPTOR TYROSINE PHOSPHATASE BETA) MONOCLONAL ANTIBODY - 2341911 2341911
      MOUSE ANTI-PHOSPHACAN (CHICKEN) (RECEPTOR TYROSINE PHOSPHATASE BETA) - 3387590 3387590
      MOUSE ANTI-PHOSPHACAN (CHICKEN) (RECEPTOR TYROSINE PHOSPHATASE BETA) - 3761083 3761083
      MOUSE ANTI-PHOSPHACAN (CHICKEN) (RECEPTOR TYROSINE PHOSPHATASE BETA) - 4008322 4008322
      MOUSE ANTI-PHOSPHACAN (CHICKEN) (RECEPTOR TYROSINE PHOSPHATASE BETA) MONOCLONAL ANTIBODY 3150379
      MOUSE ANTI-PHOSPHACAN (CHICKEN) (RECEPTOR TYROSINE PHOSPHATASE BETA) MONOCLONAL ANTIBODY 3114930
      MOUSE ANTI-PHOSPHACAN (CHICKEN) - 3991560 3991560
      MOUSE ANTI-PHOSPHACAN (CHICKEN) - 4159434 4159434
      MOUSE ANTI-PHOSPHACAN (CHICKEN) -2622640 2622640
      MOUSE ANTI-PHOSPHACAN (CHICKEN) -2657453 2657453
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      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies