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ABE1788-25UG Anti-Nicotinamide N-methyltransferase

Anti-Nicotinamide N-methyltransferase Antibody, Cat. No. ABE1788, is a highly specific rabbit polyclonal antibody that targets human Nicotinamide N-methyltransferase and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category: Epigenetics & Nuclear Function Gene Symbol: NNMT UniProt Number: P40261
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ABE1788-25UG
25 μg  
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      Replacement Information

      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1788-25UG
      DescriptionAnti-Nicotinamide N-methyltransferase
      Alternate Names
      • EC 2.1.1.1
      Background InformationNicotinamide N-methyltransferase (UniProt: P40261; also known as EC: 2.1.1.1) is encoded by the NNMT gene (Gene ID: 4837) in human. Nicotinamide N-methyltransferase is a monomeric, cytoplasmic enzyme that belong to the class I -like SAM-binding methyltransferase superfamily. It catalyzes the N-methylation of nicotinamide and other pyridines with S-Adenosyl-L-methionine (SAM)) serving as the methyl donor. It has two SAM binding sites (aa 63-64 and 142-143). Its catalytic activity is important for biotransformation of many drugs and xenobiotic compounds. Nicotinamide N-methyltransferase is predominantly expressed in the liver. A lower level of expression is seen in the kidney, lung, skeletal muscle, placenta, and heart and it is not detected in the brain or pancreas. Nicotinamide N-methyltransferase inhibition is shown to increases adipose SAM and NAD(+) levels and upregulate ornithine decarboxylase and spermidine-spermine N(1)-acetyltransferase activity and expression. (Ref.: Kraus, D et al. (2014). Nature. 508 (7495); 258-62).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Nicotinamide N-methyltransferase Antibody, Cat. No. ABE1788, is a highly specific rabbit polyclonal antibody that targets human Nicotinamide N-methyltransferase and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected Nicotinamide N-methyltransferase in 10 µg of  human kidney lysate and HEK293 cell lysate.
      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Nicotinamide N-methyltransferase in human liver, human kidney, and human pancreas tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human Nicotinamide N-methyltransferase.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects human Nicotinamide N-methyltransferase. It targets an epitope within 15 amino acids from the internal region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • NNMT
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~30 kDa observed; 29.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.
      Western Blotting Analysis: 1 µg/mL of this antibody detected Nicotinamide N-methyltransferase in 10 µg of  HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      ABE1788-25UG 04054839526916

      Documentation