Millipore Sigma Vibrant Logo

MABF2275-25UL Anti-MXRA8 Antibody, clone 9G2.D6

MABF2275-25UL
25 µL  
Prix en cours de récupération
Le prix n'a pas pu être récupéré
La quantité minimale doit être un multiple de
Maximum Quantity is
À la validation de la commande Plus d'informations
Vous avez sauvegardé ()
 
Demander le prix
Disponibilité limitée
Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

       

      Contacter le Service Clients

      Aperçu

      Replacement Information
      Description
      Catalogue NumberMABF2275-25UL
      DescriptionAnti-MXRA8 Antibody, clone 9G2.D6
      Alternate Names
      • Matrix remodeling-associated protein 8
      • Adipocyte-specific protein 3
      • Dual Ig domain-containing cell adhesion molecule
      • DICAM
      • Limitrin
      Background InformationMatrix remodeling-associated protein 8 (UniProt: Q9DBV4; also known as Adipocyte-specific protein 3, Dual Ig domain-containing cell adhesion molecule, DICAM, Limitrin, Mxra8) is encoded by the Mxra8 (also known as Asp3, Dicam) gene (Gene ID: 74761) in murine species. Mxra8 is a single-pass type I membrane protein that is highly expressed at areas of cell-cell contact and may localize to tight junctions. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 20-340), a transmembrane domain (aa 341-361), and a cytoplasmic domain (aa 362-442). Its high expression is observed in the brain where it localizes to glia limitans formed by the endfeet of astrocytes surrounding capillaries, and beneath the pia mater. It is also expressed in intercalated disks in the heart and has been detected in pancreatic alpha-cells. It modulates the activity of various signaling pathways via binding to integrin aV:b3.. Mxra8 also acts as an entry mediator for multiple emerging arthritogenic alphaviruses, including chikungunya, Ross River, Mayaro, and O nyong nyong viruses. It is shown to directly bind to chikungunya virus particles and enhances virus attachment and internalization into cells. Clone 9G2.D6 is shown to block chikungunya virus infection in multiple cell types, including primary human synovial fibroblasts, osteoblasts, chondrocytes and skeletal muscle cells. (Ref.: Zhang, R., et al. (2018). Nature. 557(7706); 570-574).
      References
      Product Information
      FormatPurified
      PresentationPurified Armenian hamster monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-MXRA8, clone 9G2.D6, Cat. No. MABF2275, is an Armenian hamster monoclonal antibody that detects MXRA8 and is tested for use in Flow Cytometry, and Inhibition, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Inhibition
      • Western Blotting
      Application NotesInhibition Activity: A representative lot inhibited Chikungunya virus ( CHIKV) infection in 3T3 cells (Zhang, R., et. al. (2018). Nature. 557(7706):570-574).

      Western Blotting Analysis: A representative lot detected MXRA8 in Western Blotting applications (Basore, K., et. al. (2019). Cell. 177(7):1725-1737; Zhang, R., et. al. (2018). Nature. 557(7706):570-574).

      Flow Cytometry Analysis: A representative lot detected MXRA8 in Flow Cytometry applications (Zhang, R., et. al. (2018). Nature. 557(7706):570-574).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFc-fusion recombinant fragment corresponding to aa 22-336 of extracellular domain of mouse MXRA8 protein.
      Epitopeextracellular domain
      Clone9G2.D6
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostArmenian Hamster
      SpecificityClone 9G2.D6 is a Armenian Hamster monoclonal antibody that detects Matrix remodeling-associated protein 8 (MXRA8). It targets an epitope within 20 amino acids from the extracellular domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Mxra8
      • Asp3
      • Dicam
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~44 kDa observed; 49.75 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U2OS cell lysate.

      Western Blotting Analysis: A 1:250 dilution of this antibody detected MXRA8 in U2OS cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF2275-25UL 04061842591961

      Documentation

      Anti-MXRA8 Antibody, clone 9G2.D6 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-MXRA8 Antibody, clone 9G2.D6 Certificats d'analyse

      TitreNuméro de lot
      Anti-MXRA8, clone 9G2.D6 - Q3504008 Q3504008

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      Cryo-EM Structure of Chikungunya Virus in Complex with the Mxra8 Receptor
      Katherine Basore 1 , Arthur S Kim 2 , Christopher A Nelson 1 , Rong Zhang 3 , Brittany K Smith 1 , Carla Uranga 4 , Lo Vang 4 , Ming Cheng 5 , Michael L Gross 5 , Jonathan Smith 4 , Michael S Diamond 6 , Daved H Fremont
      Cell  177(7)  1725-1737  2019

      Afficher le résumé
      31080061 31080061
      Mxra8 is a receptor for multiple arthritogenic alphaviruses
      Rong Zhang 1 , Arthur S Kim 1 2 , Julie M Fox 1 , Sharmila Nair 1 , Katherine Basore 3 , William B Klimstra 4 , Rebecca Rimkunas 5 , Rachel H Fong 5 , Hueylie Lin 1 , Subhajit Poddar 1 , James E Crowe Jr 6 , Benjamin J Doranz 5 , Daved H Fremont 3 , Michael S Diamond
      Nature  557(7706)  570-574  2018

      Afficher le résumé
      29769725 29769725