Millipore Sigma Vibrant Logo

382158 Anti-Histone H3, Acetylated (1-20) Rabbit pAb

Anti-Histone H3, Acetylated (1-20), rabbit polyclonal, recognizes the ~17 kDa acetylated histone H3 protein. It is validated for use in Western blotting, immunocytochemistry & immunoprecipitation.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
View Products on Sigmaaldrich.com
382158
Voir les Prix & la Disponibilité

Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
H, TRbPolyclonal Antibody

Prix & Disponibilité

Référence DisponibilitéConditionnement Qté Prix Quantité
382158-50UG
Récupération des données relatives à la disponibilité...
—
Disponibilité limitée Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

      		 Ampoule plast. 50 μg
      Prix en cours de récupération
      Le prix n'a pas pu être récupéré
      La quantité minimale doit être un multiple de
      Maximum Quantity is
      À la validation de la commande Plus d'informations
      Vous avez sauvegardé ()
       
      —
      Demander le prix
      		 —
      Description
      OverviewRecognizes the ~17 kDa acetylated histone H3 protein.
      Catalogue Number382158
      Brand Family Calbiochem®
      Application Data
      Detection of human acetylated histone H3 by immunoblotting. Samples: Acid-extract cells left untreated (lane 1) and sodium butyrate treated (lane 2). Primary antibody: Anti-Histone H3, Acetylated (1-20) Rabbit pAb (Cat. No. 382158) (0.01 µg/ml). Detection: chemiluminescence.
      References
      ReferencesBraunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
      Braunstein, M., et al. 1993. Genes Devel. 7, 592.
      Perry, C.A., et al. 1993. Biochemistry 32, 13605.
      Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
      Positive controlHeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (10-500 ng/ml, chemiluminescence)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (see comments)
      Application CommentsFor immunocytochemistry, HeLa cells were fixed with 95% ethanol/5% acetic acid and treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h. 10-500 ng/ml of antibody detected acetylated histone H3 in acid-extracted proteins from HeLa cells. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H3. Higher concentrations of antibody also detected unacetylated histone H3. Preliminary evidence from an independent library suggests that phosphorylation of Ser10 on histone H3 may disrupt epitope recognition by this antibody. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena diacetylated synthetic peptide [ARTKQTARAcKSTGGAcKAPRKQL(C)] corresponding to amino acids 1-20 of human histone H3, conjugated to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Tetrahymena
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      382158-50UG 04055977213201

      Documentation

      Anti-Histone H3, Acetylated (1-20) Rabbit pAb FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Histone H3, Acetylated (1-20) Rabbit pAb Certificats d'analyse

      TitreNuméro de lot
      382158

      Références bibliographiques

      Aperçu de la référence bibliographique
      Braunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
      Braunstein, M., et al. 1993. Genes Devel. 7, 592.
      Perry, C.A., et al. 1993. Biochemistry 32, 13605.
      Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-August-2007 RFH
      ApplicationImmunoblotting (10-500 ng/ml, chemiluminescence)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (see comments)
      Application Data
      Detection of human acetylated histone H3 by immunoblotting. Samples: Acid-extract cells left untreated (lane 1) and sodium butyrate treated (lane 2). Primary antibody: Anti-Histone H3, Acetylated (1-20) Rabbit pAb (Cat. No. 382158) (0.01 µg/ml). Detection: chemiluminescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa acetylated histone H3 protein.
      BackgroundThe acetylation of core histones regulates gene expression through alterations in the chromatin structure. Lysine acetylation in the amino-terminal domains of histones H3 and H4 can alter the topology of the DNA in chromatin and probably influence its higher-order folding.
      HostRabbit
      Immunogen speciesHuman
      Immunogena diacetylated synthetic peptide [ARTKQTARAcKSTGGAcKAPRKQL(C)] corresponding to amino acids 1-20 of human histone H3, conjugated to KLH
      IsotypeIgG
      SpeciesTetrahymena, human
      Positive controlHeLa cells
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor immunocytochemistry, HeLa cells were fixed with 95% ethanol/5% acetic acid and treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h. 10-500 ng/ml of antibody detected acetylated histone H3 in acid-extracted proteins from HeLa cells. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H3. Higher concentrations of antibody also detected unacetylated histone H3. Preliminary evidence from an independent library suggests that phosphorylation of Ser10 on histone H3 may disrupt epitope recognition by this antibody. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBraunstein, M., et al. 1996. Mol. Cell. Biol. 16, 4349.
      Braunstein, M., et al. 1993. Genes Devel. 7, 592.
      Perry, C.A., et al. 1993. Biochemistry 32, 13605.
      Lin, R., et al. 1989. J. Cell. Biol. 108, 1577.