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MABF2335-25UG Anti-HCV E2 Antibody, clone AR1B

MABF2335-25UG
25 μg  
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      Aperçu

      Replacement Information
      Description
      Catalogue NumberMABF2335-25UG
      DescriptionAnti-HCV E2 Antibody, clone AR1B
      Alternate Names
      • Hepatitis C virus envelope protein
      • gp70
      • gp68
      Background InformationHepatitis C virus (HCV) is a small (~55-65 nm), enveloped, positive-sense single-stranded RNA virus that is a causative factor for hepatitis C and hepatocellular carcinoma. HCV expresses several proteins that promote viral replication. Genome polyprotein of HCV is a core protein that packages viral RNA to form a viral nucleocapsid, and promotes virion budding. It also modulates viral translation initiation by interacting with HCV IRES and 40S ribosomal subunit. It prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) and IFN-gamma signaling pathways and by inducing human STAT1 degradation. Envelope glycoproteins E1 (aa 192-383) and E2 (aa 384-746) of HCV are single-pass type I membrane proteins that form a heterodimer by non-covalent interaction of their transmembrane domains, which is involved in virus attachment to the host cell, virion internalization through clathrin-dependent endocytosis and fusion with host membrane. The C-terminal transmembrane domain of E1 and E2 acts as a signal sequence and forms a hairpin structure before cleavage by host signal peptidase. After cleavage, the membrane sequence is retained at the C-terminus of the protein, serving as ER membrane anchor. A reorientation of the second hydrophobic stretch occurs after cleavage producing a single reoriented transmembrane domain. The E2 antigenic site localized to amino acids 412-423 is reported to be highly conserved and is considered as an ideal target for cross neutralization of HCV genotypes. Clone AR1B is derived from Fab clone D1 and is shown to bind to E2 and does not block E1E2 binding to CD81. (Ref.: Giang, E., et al. (2012). Proc. Natl. Acad. Sci. USA. 109(16); 6205-6210).
      References
      Product Information
      FormatPurified
      PresentationPurified human recombinant monoclonal antibody in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-HCV E2, clone AR1B, Cat. No. MABF2335, is a human recombinant monoclonal antibody that detects Hepatitis C virus E2 glycoprotein and is tested for use in ELISA and Neutralizing Activity.
      Key Applications
      • ELISA
      • Neutralizing
      Application NotesNeutralizing: A representative lot neutralized HCVpp-H77 in Neutralizing applications (Law, M., et. al. (2008). Nat Med. 14(1):25-7).

      ELISA Analysis: A representative lot detected HCV E2 in ELISA applications (Giang, E., et. al. (2012). Proc Natl Acad Sci USA. 109(16):6205-10; Law, M., et. al. (2008). Nat Med. 14(1):25-7).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenAntibody Fab fragment D1 expressed in E. coli, purified, and converted into full-length IgG1 molecules in CHO-K1 cells.
      EpitopeDiscontinuous; E2
      CloneAR1B
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostHuman Recombinant
      SpecificityClone AR1B is a recombinant antibody produced from Fab fragment D1, expressed in E.coli and converted into full-length IgG1 in CHO-K1 cells. It detects E2 envelope protein in Hepatitis C virus.
      IsotypeIgG1
      Species Reactivity
      • Virus
      Species Reactivity NoteHepatitis C Virus.
      Antibody TypeRecombinant Monoclonal Antibody
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight327.15 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by ELISA in lysate from 293T cells transfected with H77E1E2.

      ELISA Analysis: A x/2 dilution of this antibody detected Hepatitis C virus E2 glycoprotein in HEK293T cells transfected with H77E1E2, but not in control cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABF2335-25UG 04061842626021

      Documentation

      Anti-HCV E2 Antibody, clone AR1B FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-HCV E2 Antibody, clone AR1B Certificats d'analyse

      TitreNuméro de lot
      Anti-HCV E2, clone AR1B - Q3504017 Q3504017

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      Human broadly neutralizing antibodies to the envelope glycoprotein complex of hepatitis C virus
      Erick Giang 1 , Marcus Dorner, Jannick C Prentoe, Marlène Dreux, Matthew J Evans, Jens Bukh, Charles M Rice, Alexander Ploss, Dennis R Burton, Mansun Law
      Proc Natl Acad Sci U S A  109(16)  6205-10  2011

      Afficher le résumé
      22492964 22492964
      Broadly neutralizing antibodies protect against hepatitis C virus quasispecies challenge
      Mansun Law 1 , Toshiaki Maruyama, Jamie Lewis, Erick Giang, Alexander W Tarr, Zania Stamataki, Pablo Gastaminza, Francis V Chisari, Ian M Jones, Robert I Fox, Jonathan K Ball, Jane A McKeating, Norman M Kneteman, Dennis R Burton
      Nat Med  14(1)  25-7  2008

      Afficher le résumé
      18064037 18064037