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MAB360-25UL Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 (mouse monoclonal)

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB with more than 65 p

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Research Category: Neuroscience Research Sub-Category: Neuronal & Glial Markers Gene Symbol: GFAP, FLJ45472 UniProt Number: P14136
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MAB360-25UL
25 µL  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ch, H, M, Po, R, RbICC, IHC, IH(P), WBMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMAB360-25UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glial Fibrillary Acidic Protein Antibody, clone GA5 (mouse monoclonal)
      Alternate Names
      • GFAP
      Background InformationGlial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
      Product Information
      FormatAscites
      Control
      • Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
      PresentationAscites fluid. Liquid. Contains no preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB with more than 65 product citations.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunocytochemistry:
      A previous lot was used on cultured mammalian cells.

      Immunohistochemistry(paraffin):
      1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.

      Immunoblotting: A 1:1000 dilution of a previous lot was used.

      Optimal working dilutions must be determined by the end user
      Biological Information
      ImmunogenPurified GFAP from porcine spinal cord.
      CloneGA5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityGlial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin.
      IsotypeIgG1
      Species Reactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Pig
      • Rat
      • Rabbit
      Species Reactivity NoteReacts with Bovine, Chicken, Human, Mouse, Porcine (Pig), Rabbit and Rat. Reactivity with other species has not been determined.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
      Gene Symbol
      • GFAP
      • FLJ45472
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
      SIZE: 432 amino acids; 49880 Da
      SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
      SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
      DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander disease [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
      SIMILARITY: SwissProt: P14136 ## Belongs to the intermediate filament family.
      Molecular Weight~ 51 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Mouse brain lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Global Trade Item Number
      Référence GTIN
      MAB360-25UL 04054839337673