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MABE1669 Anti-Cas9 Antibody, NT Antibody, clone 8C1-F10

Anti-Cas9, NT, clone 8C1-F10 , Cat. No. MABE1669, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cas9/Csn1 and has been tested in Immunocytochemistry and Western Blotting.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function Gene Symbol: cas9, csn1 UniProt Number: Q99ZW2
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MABE1669
100 μg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1669
      DescriptionAnti-Cas9 Antibody, NT Antibody, clone 8C1-F10
      Alternate Names
      • CRISPR-associated endonuclease Cas9/Csn1
      • SpyCas9
      Background InformationCRISPR-associated endonuclease Cas9/Csn1 (UniProt Q99ZW2; also known as SpyCas9) is encoded by the Streptococcus pyogenes serotype M1 gene Cas9 (also known as csn1) gene (Gene ID 901176). Clustered regularly interspaced short palindromic repeats (CRISPRs) are DNA loci containing short repetitions of base sequences complementary to antecedent mobile elements and target invading nucleic acids. Each repetition is followed by short segments of spacer DNA from previous exposures to a virus. Cas9 is an essential endonuclease in Streptococcus pyogenes serotype M1's CRISPR immune system that confers resistance to foreign genetic elements such as plasmids and phages. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems, correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc), and Cas9. Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed 3'-5' exonucleolytically.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cas9, NT, clone 8C1-F10 , Cat. No. MABE1669, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cas9/Csn1 and has been tested in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected exogenously expressed full-length nuclease-dead Cas9 in transfected NIH/3T3 cells fixed with 4% paraformaldehyde and permeabilized by 0.3% Triton X-100.
      Biological Information
      ImmunogenHis-tagged recombinant Cas9 N-terminal fragment excluding the active site RuvC-I.
      Clone8C1-F10
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 8C1-F10 targets an epitope within Cas9 N-terminal region outside the active site RuvC-I.
      IsotypeIgG2bκ
      Species Reactivity
      • All
      Species Reactivity NoteStreptococcus pyogenes.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • cas9
      • csn1
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~160 kDa observed. 158.4 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH3T3 cells.

      Western Blotting Analysis: 0.25 µg/mL of this antibody detected exogenously expressed full-length nuclease-dead Cas9 in 10 µg of lysate from transfected NIH/3T3 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABE1669 04054839111723

      Documentation

      Anti-Cas9 Antibody, NT Antibody, clone 8C1-F10 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Cas9 Antibody, NT Antibody, clone 8C1-F10 Certificats d'analyse

      TitreNuméro de lot
      Anti-Cas9, NT, clone 8C1-F10 - 3900807 3900807
      Anti-Cas9, NT, clone 8C1-F10 - 4083464 4083464
      Anti-Cas9, NT, clone 8C1-F10 - 4145817 4145817
      Anti-Cas9, NT, clone 8C1-F10 - 4178988 4178988
      Anti-Cas9, NT, clone 8C1-F10 -Q2790922 Q2790922
      Anti-Cas9, NT, clone 8C1-F10 Monoclonal Antibody 3101492