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MABC593 Anti-Astrin/SPAG5, clone 8B2 Antibody

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MABC593
100 µg  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, WB, IPRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC593
      DescriptionAnti-Astrin/SPAG5, clone 8B2 Antibody
      Alternate Names
      • Sperm-associated antigen 5
      • Astrin
      • Deepest
      • Mitotic spindle-associated protein p126
      • MAP126
      Background InformationAstrin/SPAG5 is a microtubule binding and cytoskeleton associated signaling protein that plays not only a critical role in mitotic spindle and chromosome segregation but also as an important signal transducer in mTOR and GSK3 beta signaling pathways. Astrin/SPAG5 is localized in the cytoplasm and/or mitotic apparatus (in dividing cells) and centrosomes. In non-dividing cells Astrin/SPAG5 is associated with microtubules unless under cellular stress, then it is localized in stress granules. Astrin/SPAG5 is expressed in many tissues but is very high in testis and sperm and is regulated via the cell cycle highest levels are seen in prophase. Recent research has focused on Astrin/SPAG5 critical role in regulating mTORC1 and its effects on growth and survival. When cells are stress Astrin/SPAG5 inhibits mTORC1 association and recruits the mTORC1 component raptor to stress granules (SGs), thereby preventing mTORC1-hyperactivation-induced apoptosis. Because cancerous tumor cells are often stressed, this mechanism is believed to be key to their survival, thus Astrin/SPAG5 activity repression is being examined as potential anti-cancer therapy.
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2cκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Astrin/SPAG5, clone 8B2 Antibody is validated for use in Immunocytochemistry and Western Blotting and Immunoprecipitation for the detection of Astrin/SPAG5.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: A representative lot detected Astrin/SPAG5 HeLa cells (Thedieck, K., et al. (2013). Cell. 154:859-874).
      Western Blotting Analysis: A representative lot detected Astrin/SPAG5 in SiAstrin (Dr. Elisabeth Kremmer).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated Astrin/SPAG5 in SiAstrin (Dr. Elisabeth Kremmer).
      Biological Information
      ImmunogenLinear peptide corresponding to human Astrin/SPAG5.
      Clone8B2
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      IsotypeIgG2cκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SPAG5
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight134 kDa calculated
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa and A431 cells.

      Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected Astrin/SPAG5 in HeLa and A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Référence GTIN
      MABC593 04055977112658