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MABS1232 Anti-AMPK alpha 1/2 Antibody, clone 34.2

Anti-AMPK alpha Antibody, clone 34.2 is an antibody against AMPK alpha 1/2 for use in Western Blotting.

Anti-AMPK alpha 1/2 Antibody, clone 34.2 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Signaling Research Sub-Category: Kinases & Phosphatases Gene Symbol: AMPKalpha, EG:132E8.2, SNF1A, SNF1A-RA, CG3051/Dmel_CG3051, PRKAA1, PRKAA2 UniProt Number: O18645
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MABS1232
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, Dr, MWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1232
      DescriptionAnti-AMPK alpha 1/2 Antibody, clone 34.2
      Alternate Names
      • AMP-activated protein kinase alpha subunit, isoform A
      • AMP-activated protein kinase alpha subunit, isoform B
      • AMP-activated protein kinase alpha subunit, isoform C
      • EG:132E8.2 protein
      • FI03728p
      • SNF1A/AMP-activated protein kinase
      • PRKAA1
      • PRKAA2
      Background InformationAMP-activated protein kinase alpha subunit, isoform A (EC 2.7.11.16; UniProt O18645; also known as AMP-activated protein kinase alpha subunit, isoform B, AMP-activated protein kinase alpha subunit, isoform C, EG:132E8.2 protein, FI03728p, SNF1A/AMP-activated protein kinase) is encoded by the AMPKalpha (also known as EG:132E8.2, SNF1A, SNF1A-RA) gene (ORF CG3051/Dmel_CG3051; Gene ID 43904) in Drosophila melanogaster (Fruit fly) species. The yeast sucrose non-fermenting 1 (SNF1) and the AMP-activated protein kinase (AMPK) in drosphila and mammals are orthologous heterotrimeric serine-threonine kinases, each consisting of a catalytic α subunit and regulatory β and γ subunits, that sense decreased cellular energy levels when the AMP to ATP ratio rises due to a high rate of metabolism or decreased nutrient availability. AMP binding to the γ subunit results in an enhanced phosphorylation of the catalytic α subunit on an activation loop threonine and AMPK allosteric activation. Activated AMPK phosphorylates downstream targets, leading to energy conservation and inhibition of ATP consumption. For example, the high rate of ATP use in contracting muscle causes AMPK-mediated phosphorylation/inactivation of acetyl-CoA carboxylase (ACC), thereby increasing fatty acid oxidation and restoring cellular energy. AMPK functions as a positive regulator of autophagy by phosphorylating the protein kinase ULK1 that initiates autophagy as well as by inhibiting the mammalian TOR (mTOR) pathway activity via phosphorylating mTOR-binding partner raptor. In mammals, there exist two different α (encoded by the PRKAA1 and PRKAA2 gene), two different β (encoded by the PRKAB1 and PRKAB2 gene), and three different γ (encoded by the PRKAG1, PRKAG2, and PRKAG3 gene) subunits.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-AMPK alpha Antibody, clone 34.2 is an antibody against AMPK alpha 1/2 for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected AMPK alpha 1/2 in HEK293 cell lysate, rat brain homogenate, and Drosophila melanogaster S2 cell lysate (Courtesy of Professor Jay Brenman, UNC School of Medicine, USA).
      Biological Information
      ImmunogenLinear peptide corresponding to a sequence from the N-terminal catalytic domain of drosophila AMPK alpha subunit.
      EpitopeN-terminal catalytic domain.
      Clone34.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 34.2 reacts with drosophila AMPK alpha subunit, as well as AMPK catalytic subunits alpha 1 and alpha 2 of diverse mammalian species, including human, rat, and mouse.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Rat
      • Drosophila
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AMPKalpha
      • EG:132E8.2
      • SNF1A
      • SNF1A-RA
      • CG3051/Dmel_CG3051
      • PRKAA1
      • PRKAA2
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~73 kDa observed. 64.60 kDa (drosophila), 64.01/63.93/63.97 kDa (human/mouse/rat AMPK subunit alpha-1), 62.32/62.02/62.26 kDa (human/mouse/rat AMPK subunit alpha-2) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected AMPK alpha 1/2 in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABS1232 04055977344233