Typical Concentrate Volume/Concentration Factor vs. Spin Time

Concentrate Volume / Concentration Factor
	3K device		10K device		30K device		50K device		100K device
Spin time (min)	Conc. Volume (µL)	Conc. Factor (x)	Conc. Volume (µL)	Conc. Factor (x)	Conc. Volume (µL)	Conc. Factor (x)	Conc. Volume (µL)	Conc. Factor (x)	Conc. Volume (µL)	Conc. Factor (x)
5	215	2	66	7	42	12	28	18	58	9
10	114	4	35	14	23	22	20	25	19	26
15	80	6	22	22	19	27	17	30	15	33
20	62	8	20	24	17	30	15	33	13	36
30	48	10	15	31	15	32	15	36	11	41
Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume. Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=12. Shaded volumes were used for the calculation of protein recovery in the Typical Concentrate Recovery table.