Typical Concentrate Recovery Spin Conditions

Marker/Concentration	Molecular Weight	Device NMWL	Spin Time (min)	Conc. Volume (µL)	Conc. Factor (x)	Concentrate Recovery (%)
Cytochrome c (0.25 mg/mL)	12,400	3K	30	48	10	98
Cytochrome c (0.25 mg/mL)	12,400	10K	15	22	22	93
BSA (1 mg/mL)	67,000	30K	10	23	22	97
BSA (1 mg/mL)	67,000	50K	10	20	25	92
IgG (1 mg/mL)	156,000	100K	10	19	26	92
Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume, n=12. The shaded volumes were taken from the Typical Concentrate Volume/Concentration Factor vs. Spin Time table.