Chromolith® Performance RP-18 endcapped 100-4.6 mm
Column	Chromolith® Performance RP-18 endcapped 100-4.6 mm
Mobile phase	A: Acetonitrile with 0.1% TFA B: Water with 0.1% TFA
Gradient	0–10 min	15–80% A
Detection	UV 270 nm
Injection volume	2 μL
Sample	1. Nadolol 2. Metoprolol 3. Propranolol	1 mg/mL 1 mg/mL 0.5 mg/mL
Chromolith® SemiPrep RP-18 endcapped 100-10 mm
Column	Chromolith® SemiPrep RP-18 endcapped 100-10 mm
Mobile phase	A: Acetonitrile with 0.1% TFA B: Water with 0.1% TFA
Gradient	0 –10 min	15 – 80% A
Flow rate	4.7 mL/min
Detection	UV 270 nm
Injection volume	100 μL
Sample	1. Nadolol 2. Metoprolol 3. Propranolol	100 mg/mL 100 mg/mL 50 mg/mL