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70765 pET Expression System 22b - Novagen

pET vectors plus host strains with induction control

pET Expression System 22b - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70765
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      Description
      OverviewpET Expression Systems and pET
      pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

      Components: pET Expression Systems
      Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
      • 10 µg pET vector DNA (for each indicated plasmid)
      • 0.2 ml BL21 Glycerol Stock
      • 0.2 ml BL21(DE3) Glycerol Stock
      • 0.2 ml BL21(DE3)pLysS Glycerol Stock
      • 0.2 ml Induction Control Glycerol Stock

      Components: pET Expression Systems plus Competent Cells
      pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
      • 0.2 ml NovaBlue Competent Cells
      • 0.2 ml BL21(DE3) Competent Cells
      • 0.2 ml BL21(DE3)pLysS Competent Cells
      • 2 × 0.2 ml SOC Medium
      • 10 µl Test Plasmid

      These components are sufficient for up to 10 transformations in each host.

      Purification and Detection Reagents
      Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

      pET Expression System 22b
      The pET-22b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB038VM.




      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number70765
      Brand Family Novagen®
      References
      Product Information
      Components
      Fusion tagHis•Tag
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      70765-3 04055977273182

      Documentation

      pET Expression System 22b - Novagen Certificates of Analysis

      TitleLot Number
      70765

      Citations

      Title
    • Jiro Arima, et al. (2008) Streptomyces aminopeptidase P: biochemical characterization and insight into the roles of its N-terminal domain. Protein Engineering Design and Selection 21, 45-53.
    • Kimberly A. Burkhard and Angela Wilks. (2007) Characterization of the outer membrane receptor shuA from the heme uptake system of Shigella dysenteriae: substrate specificity and identification of the heme protein ligands. Journal of Biological Chemistry 282, 15126-15136.
    • Wei Cao, et al. (2007) RAET1E2, a soluble isoform of the UL16 binding protein RAET1E produced by tumor cells, inhibits NKG2D-mediated NK cytotoxicity.. Journal of Biological Chemistry 282, 18922-18928.
    • Testuro Fujii, et al. (2007) Stable supply of large amounts of human Fab from the inclusion bodies in E. coli. Journal of Biochemistry (Tokyo) 141, 699-707.
    • Mark S. McClain and Timothy L. Cover. (2007) Functional analysis of neutralizing antibodies against Clostridium perfringens epsilon-toxin. Infection and Immunity 75, 1785-1793.
    • Hisashi Nishiwaki, et al. (2007) Cloning, functional characterization, and mode of action of a novel insecticidal pore-forming toxin, sphaericolysin, produced by Bacillus sphaericus. Applied and Enviornmental Microbiology 73, 3404-3411.
    • Laura M. Roy, et al. (2007) Human polymorphic variants of the NEIL1 DNA glycosylase. Journal of Biological Chemistry 282, 15790-15798.
    • Katja Strube, Simon de Vries and Rainer Cramm. (2007) Formation of a dinitrosyl iron complex by NorA, a nitric oxide binding di-iron protein from Ralstonia eutropha H16. Journal of Biological Chemistry 282, 20292-20300.
    • Elizebeth C. Turner and Charles J. Dorman. (2007) H-NS antagonism in Shigella flexneri by VirB, a virulence gene yranscription regulator that is closely related to plasmid partition factors. Journal of Bacteriology 189, 3403-3413.
    • Ernest P. Williams, et al. (2007) Mycobacterium tuberculosis SigF regulates genes encoding cell wall-associated proteins and directly regulates the transcriptional regulatory gene phoY1. Journal of Bacteriology 189, 4234-4242.
    • Frédéric Chédin, et al. (2006) The AddAB helicase/nuclease forms a stable complex with its cognate sequence during translocation. Journal of Biological Chemistry 281, 18610-18617.
    • John F. Langenheim, et al. (2006) Two wrongs can make a right: dimers of prolactin and growth hormone receptor antagonists behave as agonists. Molecular Endocrinology 20, 661-674.
    • Emma Carmelo, et al. (2005) The unstructured N-terminal tail of ParG modulates assembly of a quaternary nucleoprotein complex in transcription repression. Journal of Biological Chemistry 280, 28683-28691.
    • Veronika Csizmok, et al. (2005) A novel 2D electrophoresis technique for the identification of intrinsically unstructured proteins. Molecular and Cellular Proteomics 5, 265-273.
    • Natalie Elia, et al. (2005) Excess of Gβe over Gqαe in vivo prevents dark, spontaneous activity of Drosophila photoreceptors. Journal of Cell Biology 171, 517-526.
    • Henri-Pierre Fierobe, et al. (2005) Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined tri-functional scaffoldin. Journal of Biological Chemistry 280, 16325-16334.
    • Laurent Loiseau, et al. (2005) Analysis of the heteromeric CsdA-CsdE cysteine desulfurase, assisting Fe-S cluster biogenesis in Escherichia coli. Journal of Biological Chemistry 280, 26760-26769.
    • Isabelle S. Lucet, et al. (2005) Identification of the structural and functional domains of the large serine recombinase tnpX from Clostridium perfringens. Journal of Biological Chemistry 280, 2503-2511.
    • Jeremy Moore, et al. (2005) Recognition of saccharides by the OpcA, OpaD and OpaB outer membrane proteins from Neisseria meningitidis. Journal of Biological Chemistry 280, 31489-31497.
    • Kyoung Joon Oh, et al. (2005) Conformational changes in BID, a pro-apoptotic BCL-2 family member, upon membrane binding: a site-directed spin labeling study. Journal of Biological Chemistry 280, 753-767.
    • Amit V. Pandey and Walter L. Miller. (2005) Regulation of 17,20 lyase activity by cytochrome b5 and by serine phosphorylation of P450c17. Journal of Biological Chemistry 280, 13265-13271.
    • Mark R. Proctor, et al. (2005) Tailored catalysts for plant cell-wall degradation: redesigning the exo/endo preference of Cellvibrio japonicus arabinanase 43A. Proceedings of the National Academy of Sciences (USA) 102, 2697-2702.
    • Annabelle Varrot, et al. (2005) Mycobacterium tuberculosis strains possess functional cellulases. Journal of Biological Chemistry 280, 20181-20184.
    • John C. Williams, et al. (2005) Crystal structure of human SCO1: Implications for redox signaling by a mitochondrial cytochrome c oxidase "assembly" protein. Journal of Biological Chemistry 15202-15211.
    • Bogdan Yatsula, et al. (2005) Identification of binding sites of EVI1 in mammalian cells. Journal of Biological Chemistry 280, 30712-30722.
    • Heyue Zhou, et al. (2005) Solution structure of AF-6 PDZ domain and its interaction with the C-terminal peptides from neurexin and Bcr. Journal of Biological Chemistry 280, 13841-13847.
    • Guoqiang Zhu, et al. (2005) Histone H1 proteins act as receptors for the 987P fimbriae of enterotoxigenic Escherichia coli. Journal of Biological Chemistry 280, 23057-23065.
    • Tao Dong, et al. (2004) HIV-specific cytotoxic T cells from long-term survivors select a unique T cell receptor. Journal of Experimental Medicine 200, 1547-1557.
    • Hiroyasu Furukawa and Eric Gouaux. (2003) Mechanisms of activation, inhibition and specifcity: crystal structures of the NMDA receptor NR1ligand-binding core. European Molecular Biology Organization Journal 22, 2873-2885.
    • Anja Seybert, et al. (2002) Biochemical characterisation of the clamp/clamp loader proteins from the euryarchaeon Archaeoglobus fulgidus. Nucleic Acids Research 30, 4329-4338.
    • Daniela Barilla, Barbara A. Lee and Nick J. Proudfoot. (2001) Cleavage/ polyadenylation factor IA associates with the carboxyl-terminal domain of RNA polymerase II in Saccharomyces cerevisiae. Procedings of the National Academy of Science 98, 445-450.
    • Jae Hyung Koo, Ick Hyun Cho and Yu Sam Kim. (2000) The malonate decarboxylase operon of Acinetobacter calcoaceticus KCCM 40902 is regulated by malonate and the transcriptional repressor MdcY. Journal of Bacteriology 182, 6382-6390.
    • Z. Lin, T. Thorsen and F.H. Arnold. (1999) Functional expression of horseradish peroxidase in E. coli by directed evolution. Biotechnology Progress (?) 15, 467-471.
    • Gavin MacBeath, Peter Kast and Donald Hilvert. (1998) Redesigning enzyme topology by directed evolution. Science 279, 1958-1961.
    • Allan J. MacKenzie-Graham, et al. (1997) Myelin protein expression is increased in lymph nodes of mice with relapsing experimental autoimmune encephalomyelitis. Journal of Immunology 159, 4601-4610.
    • Ralph H. Lambalot and Christopher T. Walsh. (1995) Cloning, overproduction, and characterization of the Escherichia coli holo-acyl carrier protein synthase. Journal of Biological Chemistry 270, 24658-24661.
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      User Protocols

      Title
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB055 pET System Manual

      Vector Map

      Title
      TB038VM pET-22b(+) Vector Map

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