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596105 THIOLYTE® Monobromobimane Reagent, Fluorescent Labeling Compound

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596105
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Overview

Replacement Information

Key Spec Table

CAS #Purity
71418-44-5≥95% by HPLC

Pricing & Availability

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596105-100MG
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      596105-250MG
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          596105-25MG
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              Description
              OverviewHas been used to label E. coli thioredoxin. Weakly fluorescent but reacts with thiols to yield highly fluorescent stable thioethers.
              Catalogue Number596105
              Brand Family Calbiochem®
              SynonymsTHIOLYTE® MB Reagent, MBB
              References
              ReferencesMansoor, M.A., et al. 1992. Anal. Biochem. 200, 218.
              Zangerle, L., et al. 1992. J. Neurochem. 59, 181.
              Agarwal, R. and Burke, M. 1991. Arch. Biochem. Biophys. 290, 1.
              Martin, R.L., et al. 1989. J. Biol. Chem. 264, 11768.
              Bonet, A., et al. 1988. Biochem. Biophys. Res. Commun. 152, 1.
              Chinn, P.C. 1985. Fed. Proc. 44, 1620.
              Product Information
              CAS number71418-44-5
              FormYellow solid
              Structure formula ImageStructure formula Image
              Quality LevelMQ100
              Applications
              Biological Information
              Purity≥95% by HPLC
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Standard Handling
              Storage +15°C to +30°C
              Protect from Light Protect from light
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C. Aqueous stock solutions have a half-life of ~4 h at room temperature (20°C).
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications
              Global Trade Item Number
              Catalogue Number GTIN
              596105-100MG 07790788051884
              596105-250MG 07790788051891
              596105-25MG 07790788051907

              Documentation

              THIOLYTE® Monobromobimane Reagent, Fluorescent Labeling Compound SDS

              Title

              Safety Data Sheet (SDS) 

              THIOLYTE® Monobromobimane Reagent, Fluorescent Labeling Compound Certificates of Analysis

              TitleLot Number
              596105

              References

              Reference overview
              Mansoor, M.A., et al. 1992. Anal. Biochem. 200, 218.
              Zangerle, L., et al. 1992. J. Neurochem. 59, 181.
              Agarwal, R. and Burke, M. 1991. Arch. Biochem. Biophys. 290, 1.
              Martin, R.L., et al. 1989. J. Biol. Chem. 264, 11768.
              Bonet, A., et al. 1988. Biochem. Biophys. Res. Commun. 152, 1.
              Chinn, P.C. 1985. Fed. Proc. 44, 1620.
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision03-April-2008 RFH
              SynonymsTHIOLYTE® MB Reagent, MBB
              DescriptionContains a reactive functional group (allylic bromide) that is capable of reacting with a wide variety of nucleophiles to form stable covalent adducts. Reacts extremely rapidly with thiol groups under physiological conditions to yield highly fluorescent products. A labeling reaction is typically carried out at room temperature for 2 to 60 min, pH 7.4, using an equivalent amount (or a small excess) of reagent relative to the amount of thiol. Material is chemically and photolytically stable and shows an absorption maxima at 370 to 385 nm and emits light at 477 to 484 nm (turquoise). Readily penetrates membranes. Useful as an alternative tool in labeling methodology, complementing the use of such well known fluorescent labeling reagents as fluorescein and rhodamine isothiocyanates (whose reactions are slower and yield products that fade relatively fast during irradiation) and fluorescamine (which reacts primarily with amine groups, but whose products also fade relatively rapidly).
              FormYellow solid
              CAS number71418-44-5
              Structure formulaStructure formula
              Purity≥95% by HPLC
              SolubilityAcetonitrile (13 mg/ml) or Methanol (25 mg/ml). Recommended stock solution is 13 mg/ml (50 mM) in acetonitrile.
              Storage Protect from light
              +15°C to +30°C
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C. Aqueous stock solutions have a half-life of ~4 h at room temperature (20°C).
              Toxicity Standard Handling
              ReferencesMansoor, M.A., et al. 1992. Anal. Biochem. 200, 218.
              Zangerle, L., et al. 1992. J. Neurochem. 59, 181.
              Agarwal, R. and Burke, M. 1991. Arch. Biochem. Biophys. 290, 1.
              Martin, R.L., et al. 1989. J. Biol. Chem. 264, 11768.
              Bonet, A., et al. 1988. Biochem. Biophys. Res. Commun. 152, 1.
              Chinn, P.C. 1985. Fed. Proc. 44, 1620.