Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Description
Overview
Native sphingolipid ceramide N-deacylase from Pseudomonas species. Hydrolyzes the N-acyl linkage between fatty acids and the sphingosine base of ceramide in various sphingolipids and gangliosides. This enzyme can also catalyze the reverse reaction and transacylation. It is not recommended for hydrolysis of ceramide. Note: 1MU = 1 milliunit.
Note: 1 mU = 1 milliunit.
Catalogue Number
567704
Brand Family
Calbiochem®
Synonyms
SCDase
References
References
Mitsutake, S., et al. 1997. Anal. Biochem. 247, 52. Sueyoshi, N., et al. 1997. J. Lipid Res. 38, 1923. Ito, M., et al. 1995. J. Biol. Chem. 270, 24370.
Product Information
Activity
≥1 unit/ml.
Unit of Definition
One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of asialo-GM₁ per min at 37°C, pH 6.0.
EC number
3.5.1.-
Form
Liquid
Formulation
In 50 mM sodium acetate buffer, 0.1% LUBROL® PX Detergent, pH 6.0.
Sphingolipid Ceramide N-Deacylase, Pseudomonas sp. Certificates of Analysis
Title
Lot Number
567704
References
Reference overview
Mitsutake, S., et al. 1997. Anal. Biochem. 247, 52. Sueyoshi, N., et al. 1997. J. Lipid Res. 38, 1923. Ito, M., et al. 1995. J. Biol. Chem. 270, 24370.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
18-September-2007 RFH
Synonyms
SCDase
Description
Native sphingolipid ceramide N-deacylase from Pseudomonas species. Hydrolyzes the N-acyl linkage between fatty acids and the sphingosine base of ceramide in various sphingolipids and gangliosides. This enzyme can also catalyze the reverse reaction and transacylation. It is not recommended for hydrolysis of ceramide. Note: 1MU = 1 milliunit.
Form
Liquid
Formulation
In 50 mM sodium acetate buffer, 0.1% LUBROL® PX Detergent, pH 6.0.
EC number
3.5.1.-
Contaminants
α- and β-N-acetylgalactosaminidase, α-fucosidase, α- and β-galactosidase, neuraminidases: none detected
Activity
≥1 unit/ml.
Unit definition
One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of asialo-GM₁ per min at 37°C, pH 6.0.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Mitsutake, S., et al. 1997. Anal. Biochem. 247, 52. Sueyoshi, N., et al. 1997. J. Lipid Res. 38, 1923. Ito, M., et al. 1995. J. Biol. Chem. 270, 24370.
