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AC112C Sheep Anti-Human IgG Antibody, Cy3 conjugate

This Sheep Anti-Human IgG, Cy3 conjugate, Cat. No. AC112C, detects human IgG and is validated for Immunocytochemistry staining of human IgG-producing B-cell hybridoma SA13.

Sheep Anti-Human IgG Antibody, Cy3 conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Secondary & Control Antibodies
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AC112C
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCShCy3Polyclonal Antibody
      Description
      Catalogue NumberAC112C
      DescriptionSheep Anti-Human IgG Antibody, Cy3 conjugate
      Background InformationImmunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins, which stage immune responses against pathogenic organisms. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fc gamma receptors which interact with sialylated Fc fragments on IgG molecules. The coupling of Cy3 to Anti-Human IgG antibody allows for the visualization of proteins by fluorescent microscopy.
      References
      Product Information
      FormatCy3
      PresentationPurified sheep polyclonal antibody Cy3 conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Sheep Anti-Human IgG, Cy3 conjugate, Cat. No. AC112C, detects human IgG and is validated for Immunocytochemistry staining of human IgG-producing B-cell hybridoma SA13.
      Key Applications
      • Immunocytochemistry
      Application NotesThis antibody is also available in unconjugated and Cy5-conjugated forms (Cat. Nos. AC112 and AC112S).
      Biological Information
      ImmunogenPurified human IgG.
      ConcentrationPlease refer to lot specific datasheet.
      HostSheep
      SpecificityBased on ELISA analysis using the unconjugated antibody (Cat. No. AC112), this antibody exhibits strongest immunoreactivity towards whole human IgG molecule (IgG>IgM>IgG Fc>IgG Fab>IgA>IgM Fc) with minimal or no cross-reactivity toward IgG from chicken, guinea pig, hamster, mouse, rabbit, and rat species.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Purification MethodAmmonium sulfate.
      Molecular Weight~150 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in human B-cell hybridoma SA13.

      Immunocytochemistry Analysis: A 1:250 dilution of this antibody immunostained 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized human B-cell hybridoma SA13, but not HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AC112C 04054839114588