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PK1010 PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb

This PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb is validated for use in ELISA, Immunoblotting for the detection of Syk (pTyr525/526).

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PK1010
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

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PK1010-50UL
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      Description
      OverviewRecognizes the ~72 kDa Syk protein phosphorylated at Tyr525/526 in human Syk or Tyr519/520 in mouse Syk in 293T cells expressing wild-type Syk and CD8.
      Catalogue NumberPK1010
      Brand Family Calbiochem®
      Application Data
      Detection of human Syk, phosphorylated on Tyr525/526, by immunoblotting. Samples: Whole cell lysate from 293T cells transfected with wild-type Syk and CD8, left untreated (left lane) or treated with phosphatase (right lane). Primary antibody: PhosphoDetect™ Anti-Syk (pTyr525/526) Rabbit pAb (Cat. No. PK1010) (1:1000). Detection: chemiluminescence.
      References
      ReferencesCoopman, P.J., et al. 2000. Nature 406, 742.
      Turner, M., et al. 2000. Immunol. Today 21, 148.
      Zang, J., et al. 2000. J. Biol. Chem. 275, 35442.
      Chu, D.H., et. al. 1998. Immunol. Rev. 165, 167.
      Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol. 9, 528.
      Kurosaki, T. 1997. Curr. Opin. Immunol. 9, 309.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      Positive control293T cells transfected with wild-type Syk and CD8
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (1:1000)
      Immunoblotting (1:1000)
      Application CommentsTyr525/526 represent the phosphorylation sites in human Syk, while Tyr519/520 are the equivalent phosphorylation sites in mouse Syk. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr525/526 phosphorylation sites of human Syk
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsDo not aliquot.
      Global Trade Item Number
      Catalogue Number GTIN
      PK1010-50UL 04055977227017