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17-125 PTP Assay Kit 1

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17-125
1 kit  Kit capacity: 100 assays
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      Overview

      Key Spec Table

      Key ApplicationsAnalytes AvailableDetection Methods
      PAPTP Colorimetric
      Description
      Catalogue Number17-125
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPTP Assay Kit 1
      Product Information
      Components
      • Tyrosine Phosphopeptide (RRLIEDAEpYAARG] (Cat.# 12-217)
      • Malachite Green Solution A (Cat.# 20-105)
      • Malachite Green Additive (Cat.# 20-104)
      • Phosphate Standard (Cat.# 20-103)
      • pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
      • pNPP Tyr Assay Buffer (Cat.# 20-180)
      • 96-well microtiter plate
      Detection methodColorimetric
      Quality LevelMQ100
      Applications
      ApplicationPTP Assay Kit 1 can be used to detect PTP-1B activity by dephosphorylation of the phosphopeptide (RRLIEDAEpYAARG) using malachite green detection, or by hydrolysis of pNPP.
      Key Applications
      • Phosphatase Assay
      Biological Information
      Analytes Available
      • PTP
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
      Gene Symbol
      • PTPN1
      • PTP-1B
      • PTP1B
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P18031 # May play an important role in CKII- and p60c-src-induced signal transduction cascades (By similarity).
      SIZE: 435 amino acids; 49967 Da
      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.
      PTM: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
      SIMILARITY: SwissProt: P18031 ## Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. & Contains 1 tyrosine-protein phosphatase domain.
      Product Usage Statements
      Quality Assuranceroutinely evaluated by malachite green and pNPP hydrolysis assays to detect PTP-1B (Catalog # 14-109 or Catalog # 14-358) activity by dephosphorylation of the phosphopeptide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Packaging Information
      Material Size1 kit
      Material PackageKit capacity: 100 assays
      Global Trade Item Number
      Catalogue Number GTIN
      17-125 04053252735929