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33-047 PI 3-Kinase 3-step HTRF™ Assay

The PI 3-Kinase 3-Step HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format.

PI 3-Kinase 3-step HTRF™ Assay MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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33-047
10,000 wells  Kit capacity: 10,000 wells
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      Overview

      Replacement Information
      Description
      Catalogue Number33-047
      Brand Family Upstate
      Trade Name
      • HTRF
      • Upstate
      DescriptionPI 3-Kinase 3-step HTRF™ Assay
      References
      Product Information
      Components
      • 4X Reaction Buffer
      • PIP2 (substrate, 1mM stock)
      • DM A Reaction Buffer
      • DM B Reaction Buffer
      • DM C Reaction Buffer
      • BPIP3
      • Stop Reagent
      Detection methodHTRF
      Quality LevelMQ100
      Applications
      ApplicationThe PI 3-Kinase 3-Step HTRF Assay is a high-performance assay kit that provides a universal method for testing all Class I PI3-Kinases in a homogeneous format.
      Key Applications
      • Kinase Assay
      Biological Information
      Species Reactivity
      • Human
      • Mouse
      Analytes Available
      • PI 3-Kinase
      Entrez Gene Number
      Entrez Gene SummaryPhosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
      Gene Symbol
      • PIK3CA
      • MGC142163
      • PI3K
      • MGC142161
      • p110-alpha
      Target Sub-FamilyPI3K/PI4K
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42336 # Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.
      SIZE: 1068 amino acids; 124284 Da
      SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3 (By similarity).
      DISEASE: SwissProt: P42336 # Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500]. & Defects in PIK3CA are associated with breast cancer [MIM:114480]. & Defects in PIK3CA are associated with epithelial ovarian cancer (EOC) [MIM:604370].
      SIMILARITY: SwissProt: P42336 ## Belongs to the PI3/PI4-kinase family. & Contains 1 C2 domain. & Contains 1 PI3K/PI4K domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions12 months at -70°C
      Packaging Information
      Material Size10,000 wells
      Material PackageKit capacity: 10,000 wells
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      33-047 04053252618888

      Documentation

      PI 3-Kinase 3-step HTRF™ Assay SDS

      Title

      Safety Data Sheet (SDS) 

      PI 3-Kinase 3-step HTRF™ Assay Certificates of Analysis

      TitleLot Number
      PI3 Kinase 3-Step Assay Reagent Kit (10000 wells) - 2463891 2463891
      PI3 Kinase 3-Step Assay Reagent Kit (10000 wells) - 2016723 2016723
      PI3 Kinase 3-Step Assay Reagent Kit (10000 wells) - 2031823 2031823
      PI3 Kinase 3-Step Assay Reagent Kit (10000 wells) - 2038588 2038588
      PI3 Kinase 3-Step Assay Reagent Kit (10000 wells) - 2038605 2038605
      PI3 Kinase 3-Step Assay Reagent Kit (10000 wells) - 2052255 2052255
      PI3 Kinase 3-Step Assay Reagent Kit (10000 wells) - 2109234 2109234
      PI3 Kinase 3-Step Assay Reagent Kit (10000 wells) - 2117680 2117680
      PI3 Kinase 3-Step Assay Reagent Kit (10000 wells) - 2117682 2117682
      PI3 Kinase 3-Step Assay Reagent Kit (10000 wells) - 2191767 2191767
      Show more

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      33-040 PI 3-Kinase 3-step HTRF™ Assay Show Pricing & Availability
      33-041 PI 3-Kinase 3-step HTRF™ Assay Show Pricing & Availability

      Alternative Format

      Catalogue Number Description  
      33-036 PI 3-Kinase HTRF™ Assay; 10,000 wells Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
      Learn More >>

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      HTRF™

      Categories

      Life Science Research > Protein Detection and Quantification > Activity Assays > Kinase Assays
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Enzyme Assay Kits and Reagents