Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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454850
Sigma-AldrichMAP Kinase 2, Mouse, Recombinant, E. coli
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Description
Overview
Recombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein kinase cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active.
Catalogue Number
454850
Brand Family
Calbiochem®
Synonyms
ERK2, MAPK2, p42MAPK
References
References
Fukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79. Wu, J., et al. 1993. Mol. Cell. Biol.13, 4539.
Product Information
Activity
≥5000 units/ml
Unit of Definition
One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein per min at 30°, pH 7.5.
Form
Liquid
Formulation
In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM Na₂-EDTA, 50% glycerol, 0.01% BRIJ® 35 detergent, pH 7.5.
MAP Kinase 2, Mouse, Recombinant, E. coli Certificates of Analysis
Title
Lot Number
454850
References
Reference overview
Fukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79. Wu, J., et al. 1993. Mol. Cell. Biol.13, 4539.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
07-August-2008 RFH
Synonyms
ERK2, MAPK2, p42MAPK
Description
Recombinant, mouse MAP Kinase 2 expressed in E. coli. A serine/threonine protein kinase that acts at the end of a protein cascade linking signals from cell surface receptor tyrosine kinases to cytoplasmic and nuclear events. This protein was co-expressed with MEK and is phosphorylated and active.
Form
Liquid
Formulation
In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM Na₂-EDTA, 50% glycerol, 0.01% BRIJ® 35 detergent, pH 7.5.
Recommended reaction conditions
Note: This protocol is provided only as a general guide. Researchers should standardize this assay for their own specific needs and should consult published literature.Suggested Protocol to Assay for MAPK Activators
1. Combine the following on ice:
4.0 µl 250 mM HEPES, pH 7.5, 100 mM Mg(OAc)2, 500 µM ATP
2.0 µl MAP Kinase
1-29 µl purified MEK (cell lysates or fractions from chromatography)
2.0 µl [γ-32P]ATP (1.0 µCi/µl)
add enough distilled H2O to produce a final volume of 40 µl.
2. Incubate at 30°C for 30 min.
3. Stop the reaction by adding 40 µl of 2X SDS-PAGE sample buffer.
4. The phosphorylated MAPK will show as a signal at ~45 kDa by autoradiography.
Phosphorylating Protein Substrates with Phosphorylated MAPK
1. Combine the following on ice:
5.0 µl 500 mM Tris-HCl (pH 7.5), 62.5 mM β-glycerol phosphate, 50 mM MgCl2, 5 mM EGTA, 250 µM NaF, 5 mM DTT, and 2.5 mM sodium vanadate
2 µg experimental protein or Myelin Basic Protein Peptide Substrate (Cat. No. 475920)
1.0 µl phosphorylated MAPK
2.0 µl [γ-32P]ATP (1.25 mM,100 µCi/µmoll)
add enough distilled H2O to produce a final volume of 25 µl.
2. Incubate at 30°C for 30 min.
3. Stop the reaction by spotting 20 µl of each reaction mixture onto a 1.5 cm2 piece of Whatman P81 paper.
4. Wash the squares 7X for 5 min with phosphoric acid (1% w/v).
5. Quantitate the 32P incorporation in a liquid scintillation counter.
Purity
≥90% by SDS-PAGE
Contaminants
MEK, phosphatase, protease: none detected.
Activity
≥5000 units/ml
Unit definition
One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein per min at 30°, pH 7.5.
Storage
Avoid freeze/thaw ≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Toxicity
Standard Handling
References
Fukunaga, K., and Miyamoto, E. 1998. Mol. Neurobiol. 16, 79. Wu, J., et al. 1993. Mol. Cell. Biol.13, 4539.