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14-550M MAP Kinase 2/Erk2 Protein, active, Human, 250 µg

Active, N-terminal GST tagged, recombinant, full-length, human MAP Kinase 2/Erk2. Activated with MEK1. For use in Kinase Assays.

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14-550M
250 µg  Also available in 10 μg size (cat#14-550) and in bulk (cat# 14-550-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-550M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMAP Kinase 2/Erk2 Protein, active, Human, 250 µg
      OverviewN-terminal GST tagged, recombinant, full-length, human MAP Kinase 2/Erk2, Activated with MEK1 (14-429).
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal GST tagged, recombinant, full-length, human MAP Kinase 2/Erk2. Activated with MEK1. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed in E. coli
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
      Gene Symbol
      • MAPK1
      • p40
      • PRKM2
      • MAPK2
      • PRKM1
      • p42-MAPK
      • p38
      • p41
      • ERK
      • ERK-2
      • ERT1
      • P42MAPK
      • p41mapk
      • ERK2
      Protein TargetMAPK2
      Purification MethodGlutathione agarose; Ni2+/NTA agarose
      Target Sub-FamilyCMGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      COFACTOR: Magnesium (By similarity).
      SIZE: 360 amino acids; 41390 Da
      SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
      DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight67.8kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceUsed to phosphorylate MBP (13-104)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-550) and in bulk (cat# 14-550-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-550M 04053252330902

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      Catalogue Number Description  
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      Categories

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      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases