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110218 Gram's decolorizing solution

for the Gram staining method

Gram's decolorizing solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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110218
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Overview

Replacement Information

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1102180500
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      		 Plastic bottle 500 ml
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      1102182500
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          		 Plastic bottle 2.5 l
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          Description
          Catalogue Number110218
          DescriptionGram's decolorizing solution
          OverviewGram’s decolorizing solution - for the Gram staining method, is a ready-to-use solution used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution (Cat. no.109218) - an aniline dye - in the first step. After the treatment with iodine solution (Lugol’s solution stabilized with PVP, Cat no.100567), a dye-iodine complex will form. During the decolorizing step (Gram’s decolorizing solution, Cat. no. 110218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution (Gram's safranine solution (Cat.no. 109217) and appear pink to red.
          A bottle with 500 ml Gram's decolorizing solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Cat. no. 111885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU).
          References
          Product Information
          HS Code3814 00 90
          Quality LevelMQ400
          Applications
          Applicationfor the Gram staining method
          Biological Information
          Physicochemical Information
          Density0.79 g/cm3 (20 °C)
          Flash point-10 °C
          Ignition temperature>425 °C
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Hazard Pictogram(s) Hazard Pictogram(s)
          Hazard Pictogram(s)
          Hazard Statement(s)H225: Highly flammable liquid and vapour.
          H319: Causes serious eye irritation.
          H336: May cause drowsiness or dizziness.
          Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
          P233: Keep container tightly closed.
          P240: Ground and bond container and receiving equipment.
          P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
          P242: Use non-sparking tools.
          P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
          P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
          Signal WordDanger
          Storage class3 Flammable liquids
          WGKWGK 1 slightly hazardous to water
          Disposal1
          Strongly contaminated halogen-free organic solvents: container A.
          Safety Information
          Categories of dangerhighly flammable, irritant
          Product Usage Statements
          Storage and Shipping Information
          StorageStore at +15°C to +25°C.
          Packaging Information
          Transport Information
          Declaration (railroad and road) ADR, RIDUN 1993 , 3, II
          Declaration (transport by air) IATA-DGRUN 1993 , 3, II
          Declaration (transport by sea) IMDG-CodeUN 1993 , 3, II
          Supplemental Information
          Specifications
          Suitability for microscopypasses test
          Staphylococcus aureusdark blue-violet
          E.colipink to red
          Global Trade Item Number
          Catalogue Number GTIN
          1102180500 04022536122663
          1102182500 04022536557694

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          IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Gram-Bacteria Staining