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SCC195 CT-2A-Luc Mouse Glioma Cell Line

CT-2A-Luc mouse glioma cell line is a valuable mouse model for therapeutic research on brain malignancies.

SCC195
≥1X10⁶ cells/vial   
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      Overview

      Description
      Catalogue NumberSCC195
      DescriptionCT-2A-Luc Mouse Glioma Cell Line
      OverviewThe CT-2A-Luc cell line is derived from a sub-cutaneous, non-metastatic murine glioma (astrocytoma) and is stably transduced with a firefly luciferase-EGFP reporter. CT-2A-Luc cells are marked by high levels of complex gangliosides and low distribution of the anti-angiogenic ganglioside GM3, as well as deficiency in the tumor suppressor PTEN/TSC2, a characteristic present in up to 70% of human high-grade glioma cell lines (2,3). CT-2A tumors are wild-type for p53 and recapitulate several features of human high-grade glioma, including high mitotic index and cell density, nuclear polymorphism, hemorrhage, pseudopalisading necrosis, and microvascular proliferation (4,5).

      Source:
      The parental CT-2A cell line was generated from a malignant astrocytoma formed via implantation of the carcinogen 20-methylcholanthrene in the cerebrum of a C57BL/6J mouse (6). The tumor was maintained through serial intracranial transplants prior to cell line isolation. The CT-2A-Luc luciferase cell line was clonally derived from CT-2A cells transduced with a lentiviral vector harboring a firefly luciferase (Fluc)-IRES-GFP cassette under control of the CMV promoter; cells were subsequently sorted for EGFP expression (7).
      Alternate Names
      • CT-2A Luciferase
      • CT2A Luciferase
      Background InformationGlioblastomas are among the most aggressive forms of cancer, associated with low treatment efficacy and poor survival. Recurring glioblastomas are often resistant to first-line chemotherapies (1). There is much interest in studying drug-resistant forms of glioblastomas in the effort to develop effective therapies.

      References:
      1. Weller M, Cloughesy T, Perry JR, and Wick W (2013) Neuro Oncol 15(1): 4-27.
      2. Seyfried TN, Mukherjee P (2010) J Oncol 2010:961243 doi.10.1155/2010/961243.
      3. Cotterchio M, Seyfried TN (1994) J Lipid Res 35(1): 10-14.
      4. Binello E, Qadeer ZA, Kothari HP, Emdad L, Germano IM (2012) J Cancer 3: 166-174.
      5. Martinez-Murillo R, Martinez A (2007) Histol Histopathol 22(12): 1309-1326.
      6. Zimmerman HM and Arnold H. (1941) Cancer Res 1(12): 919-938.
      7. Huysentruyt LC, Mukherjee P, Banerjee D, Shelton LM, Seyfried TN (2008) Int J Cancer 123(1): 73-84.
      Applications
      ApplicationCT-2A-Luc mouse glioma cell line is a valuable mouse model for therapeutic research on brain malignancies.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Product Usage Statements
      Quality Assurance• Each vial contains 1X10⁶ viable cells.
      • Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Global Trade Item Number
      Catalogue Number GTIN
      SCC195 04054839573736