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14-347 CHK2 Protein, active, 10 µg

Active, recombinant human CHK2, residues 5-543, containing N-terminal GST & C-terminal His-6 tags, for use in Kinase Assays.

CHK2 Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-347
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-347M when ordering the 250 µg size.
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      Overview

      Replacement Information
      Description
      Catalogue Number14-347
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionCHK2 Protein, active, 10 µg
      OverviewRecombinant human CHK2, residues 5-543, containing N-terminal GST and Cterminal His6 tags
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, recombinant human CHK2, residues 5-543, containing N-terminal GST & C-terminal His-6 tags, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceExpressed in E. coli
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryIn response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • CHEK2
      • HuCds1
      • CHK2
      • LFS2
      • RAD53
      • bA444G7
      • CDS1
      • OTTHUMP00000028871
      • PP1425
      • Cds1
      Protein TargetCHK2
      Purification Methodglutathione-agarose and Ni2+/NTA-agarose
      Target Sub-FamilyCAMK
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O96017 # Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'.
      COFACTOR: Magnesium.
      SIZE: 543 amino acids; 60915 Da
      SUBCELLULAR LOCATION: Isoform 2: Nucleus. Note=Isoform 10 is present throughout the cell. & Isoform 4: Nucleus. & Isoform 7: Nucleus. & Isoform 9: Nucleus. & Isoform 12: Nucleus.
      TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.
      DISEASE: SwissProt: O96017 # Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. & Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807]. & Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500].
      SIMILARITY: SwissProt: O96017 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily. & Contains 1 FHA domain. & Contains 1 protein kinase domain.
      Molecular Weight89.6kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of the CHKtide substrate peptide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -70°C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-347M when ordering the 250 µg size.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      14-347 04053252507373

      Documentation

      CHK2 Protein, active, 10 µg SDS

      Title

      Safety Data Sheet (SDS) 

      CHK2 Protein, active, 10 µg Certificates of Analysis

      TitleLot Number
      CHK2, active - 1832293-A 1832293-A
      CHK2, active - D8EN015U-C D8EN015U-C
      CHK2, active - D8EN015U-F D8EN015U-F

      References

      Reference overviewPub Med ID
      Linkage of ATM to cell cycle regulation by the Chk2 protein kinase.
      Matsuoka, S, et al.
      Science, 282: 1893-7 (1998)  1998

      Show Abstract       		9836640 9836640

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      14-347M CHK2 Protein, active, 250 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases