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SCC160 CFBE41o- 6.2 WT-CFTR Human CF Bronchial Epithelial Cell Line

CFBE41o- 6.2 WT-CFTR human CF bronchial epithelial cell line is used to study the relationship between CFTR mRNA expression and Cl transport function.

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Key Applications: Cell Culture, Cell Based Assays
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SCC160
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      CULT, Cell Based Assays
      Description
      Catalogue NumberSCC160
      DescriptionCFBE41o- 6.2 WT-CFTR Human CF Bronchial Epithelial Cell Line
      Alternate Names
      • CFBE41o
      • CF41o
      • CFBE
      Background InformationCystic Fibrosis (CF) is a lethal autosomal recessive disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene which functions as a cAMP-activated and phosphorylated-regulated Cl channel. The predominant mutation in the CFTR gene is a trinucleotide deletion that results in loss of a phenylalanine at amino acids 508 (ΔF508) in the CFTR protein. This mutation accounts for ~66% of all CF alleles (1).

      CFBE41o- 6.2 WT-CFTR Human CF Bronchial Epithelial Cell line is a subclone derived from the electroporation of the parental CFBE41o- cell line with an Epstein-Barr virus (EBV)-based episomal pCEP4β vector containing the 6.2 kb full length wtCFTR cDNA and a Hygromycin B resistance gene (1). The 6.2kb wtCFTR cDNA contains both the 5’ and 3’ UTR sequences that are known to affect translational efficiency and mRNA stability. The parental CFBE41o- is a CF human bronchial epithelial cell line, derived from a CF patient homozygous for the ΔF508 CFTR mutation and immortalized with the origin-of-replication defective SV40 plasmid (pSVori-) (1).

      In CFBE41o- 6.2 WT-CFTR cells, the levels of vector-driven wt-CFTR mRNA were found to be significantly higher than endogenous CFTR mRNA levels in normal 16HBE14o- bronchial epithelial cell (Cat. No. SCC150). However, cAMP-dependent Cl currents generated in CFBE41o- 6.2 WT-CFTR cells were not as high as those observed in 16HBE14o- cells, which express endogenous CFTR. Established CF bronchial epithelial cell lines that are complemented with either wild-type or ΔF508CFTR mRNA would help provide insights into the relationship between transgene-derived CFTR mRNA expression and rescue of cAMP-dependent Cl transport function.

      References:
      1. Illek B, Maurisse R, Wahler L, Kunzelmann K, Fischer H, Gruenert DC. (2008) Cl transport in complemented CF bronchial epithelial cells correlates with CFTR mRNA expression levels. Cell Physiol Biochem 22(1-4): 57-68.
      References
      Product Information
      HS Code3002 59 00
      Applications
      ApplicationCFBE41o- 6.2 WT-CFTR human CF bronchial epithelial cell line is used to study the relationship between CFTR mRNA expression and Cl transport function.
      Key Applications
      • Cell Culture
      • Cell Based Assays
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Epithelial Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
      • Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      • Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC160 04054839480478